桑属植物总RNA的提取及花青素合成基因MCHS的鉴定  

Extraction of Total RNA and Identification of Anthocyanin Biosynthesis Gene MCHS in Morus Alba

作　　者：李姣玉 邢玉薪 姚悦璠 刘开创 康少博 杨艳[1] Li Jiaoyu;Xing Yuxin;Yao Yuefan;Liu Kaichuang;Kang Shaobo;Yang Yan(Chemical and Biological Engineering College,Taiyuan University of Science and Technology,Taiyuan 030006,Shanxi,China;College of Life Science,Fujian Agriculture and Forestry University,Fuzhou 350000,Fujian,China;College of Life Information,Harbin Medical University,Harbin 150000,Heilongjiang,China)

机构地区：[1]太原科技大学化学与生物工程学院,山西太原030006 [2]福建农林大学生命科学学院,福建福州350000 [3]哈尔滨医科大学生命信息学院,黑龙江哈尔滨150000

出　　处：《农业技术与装备》2022年第1期9-11,共3页Agricultural Technology & Equipment

基　　金：项目名称山西省大学生创新创业计划训练项目(20210472);太原科技大学校级创新创业计划训练项目(XJ2021053);太原科技大学校级教学改革项目(202016)。

摘　　要：桑属植物细胞中含大量蛋白酶及多糖等生物功能性分子。以新鲜的桑叶、桑果为试验材料,应用Trizol法提取总RNA。试验表明,使用Trizol法配合氯仿—苯酚试剂提取RNA操作简单,且能够有效去除桑叶中多糖和多酚类等次生物质,从而得到高质量的RNA,以获得的RNA为模板,设计简并引物,成功从桑叶中鉴定出花青素合成的关键酶基因MCHS基因。该试验为分子水平研究桑科类黄酮代谢机制、以及桑葚功能性食品的开发等提供了一定的理论依据。Mulberry cells contain a large number of proteases and polysaccharides and other biological functional molecules,using fresh mulberry leaves,mulberry(fruit),the total RNA was isolated by Trizol combined with Chloroform-phenol.Purity and concentration of isolated RNA indicated that polysaccharide,phenol,and other biological molecules could be effectively eliminated in the mulberry plant cell by this method.Using the high-quality RNA as template,key genes of MCHS for anthocyanin synthesis was successfully identified by degenerate RT-PCR.The experimental results provide an experimental basis for the molecular level research on mechanism of flavonoids and the breeding of new varieties.

关 键 词：桑叶 RNA TRIZOL法 RT-PCR 花青素合成酶 

分 类 号：S188[农业科学—农业基础科学]