3种外源物对匍匐剪股颖耐盐性的影响  

Effects of three exogenous substances on the salt tolerance of creeping bentgrass

作　　者：王彬彬 李秋果 于景金 杨志民 WANG Binbin;LI Qiuguo;YU Jingjin;YANG Zhimin(College of Agro-grassland Science,Nanjing Agricultural University,Nanjing 210095,Jiangsu,China)

机构地区：[1]南京农业大学草业学院,江苏南京210095

出　　处：《草业科学》2022年第3期474-484,共11页Pratacultural Science

基　　金：科技部科技基础资源调查专项“南方草地牧草资源调查(2017FY100603)”;江苏省林业科技创新与推广项目“林下种草及林草畜复合经营示范推广(LYKJ[2021]29)”。

摘　　要：为研究外源物对匍匐剪股颖(Agrostis stolonifera)盐胁迫的缓解作用,以匍匐剪股颖品种‘L-93’为研究对象,通过盆栽试验探究盐胁迫下叶面喷施硫酸钾(K_(2)SO_(4))、抗坏血酸(As A)和甜菜碱(GB)对草坪质量(TQ)、叶片相对含水量(RWC)、电解质渗漏率(EL)、丙二醛(MDA)含量、离子含量和抗氧化物酶活性的影响。结果表明,盐胁迫显著降低了‘L-93’的TQ、RWC和抗氧化酶活性(P <0.05),显著提高了其EL和MDA含量(P <0.05),造成离子平衡失调。但是,喷施K_(2)SO_(4)和As A可以缓解盐胁迫对匍匐剪股颖造成的负面影响,显著提高盐胁迫下匍匐剪股颖的RWC (P <0.05),显著降低叶片EL和Na^(+)含量的积累(P <0.05),提高叶片K^(+)含量,从而降低盐胁迫下匍匐剪股颖叶片的Na^(+)/K^(+)值。此外,外源喷施K_(2)SO_(4)和As A还能在一定程度上提高过氧化氢酶(CAT)活性,增强抗氧化能力,进而提高盐胁迫下匍匐剪股颖的耐盐性。外源喷施GB可显著提高CAT活性(P <0.05),并降低匍匐剪股颖叶片Na^(+)/K^(+),但对TQ和MDA含量的影响差异不显著(P> 0.05),总体效果不如K_(2)SO_(4)和As A。In this study, we examined the ability of three exogenous substances to alleviate salt stress in the creeping bentgrass(Agrostis stolonifera) cultivar ‘L-93’. A pot experiment was conducted to investigate the effects of potassium sulfate(K_(2)SO_(4)), ascorbic acid(As A), and glycine betaine(GB) on the turf quality(TQ), relative water content(RWC),electrolyte leakage(EL), malonaldehyde(MDA) and ion contents, and antioxidant enzyme activity of the creeping bentgrass under salt stress conditions. Salt stress significantly reduced the TQ, RWC, and antioxidant enzyme activity of‘L-93’(P < 0.05) and significantly increased the EL and MDA contents(P < 0.05), resulting in an ion imbalance. However,the application of K_(2)SO_(4)and As A alleviated the negative effects of salt stress, significantly increasing RWC(P < 0.05),significantly reducing EL and the accumulation of Na^(+) in leaves(P < 0.05), and increasing the K^(+)content of leaves, thereby reducing the Na^(+)/K^(+)imbalance. In addition, exogenous spraying of K_(2)SO_(4)and As A improved the catalase(CAT) activity in and salt tolerance of the creeping bentgrass, while also enhancing its antioxidant capacity, under salt stress. Exogenous GB application significantly increased CAT activity and decreased Na^(+)/K^(+) values in creeping bentgrass leaves(P < 0.05) but had no significant effect on TQ or MDA content(P > 0.05);the overall effect was inferior to that of K_(2)SO_(4)and As A.

关 键 词：剪股颖 盐胁迫 外源物质 硫酸钾 抗坏血酸 甜菜碱 缓解盐害 

分 类 号：S688.4[农业科学—观赏园艺]