光照强度对条斑紫菜贝壳丝状体培养的影响  

Influence of distinctive light intensities on the conchocelis of Neopyropia yezoensis

作　　者：尚浩江 周伟[2] 胡传明[2] 申欣 朱朋昌 李帅[3] SHANG Hao-jiang;ZHOU Wei;HU Chuan-ming;SHEN Xin;ZHU Peng-chang;LI Shuai(Jiangsu Provincial Key Laboratory of Marine Biotechnology,Jiangsu Ocean University,Lianyungang 222000,China;Jiangsu Marine Fisheries Research Institute,Nantong 226007,China;Shanghai Ocean University,Shanghai 201306,China)

机构地区：[1]江苏海洋大学江苏省海洋生物技术重点实验室,江苏连云港222000 [2]江苏省海洋水产研究所,江苏南通226007 [3]上海海洋大学,上海201306

出　　处：《海洋科学》2022年第2期37-45,共9页Marine Sciences

基　　金：江苏省种业振兴项目(JBGS(2021)140);江苏省现代农业产业技术体系(JATS[2021]393、394、395,JATS[2020]371、372、373);南通市科技计划项目(MS22021016,JC2021087);江苏省研究生科研与实践创新计划项目(JSCX19_0984)。

摘　　要：光照与温度对条斑紫菜(Neopyropia yezoensis)贝壳丝状体生长发育与壳孢子放散水平均具有重要影响。因温度对光强的协同效应以及各培养阶段对光强的需求不同,本文仅改变培养光强,研究了不同光照强度对条斑紫菜贝壳丝状体着生、生长、发育及壳孢子放散的影响,为育苗阶段光照强度管理提供数据支撑。在16℃下采用1.3~25.0μmol/(m^(2)·s)5个光照强度梯度,接种条斑紫菜Y-9101丝状体于文蛤壳并培养至壳孢子放散,利用光学与电子显微镜、色差计与叶绿素荧光仪等,测量丝状体的着生效率、水平生长速率、纵向生长率、色度值、光合活性及壳孢子放散量。结果显示,贝壳丝状体着生效率与水平生长速率均呈现先上升后下降趋势,其中18.8μmol/(m^(2)·s)下着生效率最高,达到5.6%,6.3μmol/(m^(2)·s)下水平生长速率最大,达到86.6μm/d,纵向生长速率随光强增加而增加。6.3~18.8μmol/(m^(2)·s)下贝壳丝状体最终生长深度趋于一致、藻斑颜色均为黑色,且壳孢子放散总量高于其他处理组。25.0μmol/(m^(2)·s)下贝壳丝状体藻斑颜色偏浅黄,且壳孢子放散量较低,其最大光化学量子效率低于6.3~18.8μmol/(m^(2)·s)处理组,呈现光能利用效率下降的趋势。综上所述,6.3~18.8μmol/(m^(2)·s)为条斑紫菜贝壳丝状体生长的适宜光照强度。Some of the crucial factors that influence the growth,development,and conchospore release of Neopyropia yezoensis shell conchocelis are the light and the temperature.As a consequence of their synergistic effects,as well as the demand for distinctive light intensities at varied cultivation stages,the study of their influence on vari-ous cultivation aspects of N.yezoensis shell conchocelis was carried out by fluctuating the light intensities to ob-serve the implantation,growth,development,and the conchospores release.This research would promote the ad-ministration of light intensity in the shell conchocelis stage of N.yezoensis cultivation.For the analysis,the N.yezoensis conchocelis of Y-9101 strain was implanted on the clamshells at five distinct light intensities ranging be-tween 1.3–25.0μmol/m^(2)/s at 16℃and was cultured until the conchospores were released.Various observation parameters of the shell conchocelis,such as the growth efficiency,horizontal growth rate,growth depth,chromatic value,photosynthetic activity,and the release amount of conchospores were measured and analyzed by the optical microscope,the scanning electron microscope,the colorimeter,and the chlorophyll fluorometer.The results dem-onstrated an upward trend initially followed by a downward course in both the implantation efficiency and the horizontal growth rate of N.yezoensis shell conchocelis.The highest implantation efficiency of 5.6%was measured at 18.8μmol/m^(2)/s,and the highest horizontal growth rate of 86.6μm/d was measured at 6.3μmol/m^(2)/s.The longi-tudinal growth rate proliferated with increased light intensity.The final growth depth of shell conchocelis with the distinctive light intensities ranged between 6.3–18.8μmol/m^(2)/s was uniform,and the shell color spots appeared black.Moreover,the total release amount of conchospores was higher in comparison to the other groups.However,the color of the shell conchocelis spots at 25.0μmol/m^(2)/s appeared slightly yellow.In addition,the release amount of conchospores,actua

关 键 词：光照强度 条斑紫菜(Neopyropia yezoensis) 贝壳丝状体 光合活性 生长速率 

分 类 号：S197.3[农业科学—农业基础科学]