恩诺沙星、磺胺嘧啶和呋喃西林对共培养的萱藻丝状体和膨胀色球藻生长的影响  

Effects of enrofloxacin,sulfadiazine and nitrofurazone on the growth of filaments of Scytosiphon lomentaria and Chroococ-cus turgidus in the co-cultured condition

作　　者：刘德举 张敏[1] 庄英瑞 毕萌 宫相忠[1] LIU De-ju;ZHANG Min;ZHUANG Ying-rui;BI Meng;GONG Xiang-zhong(College of Marine Life Sciences,Ocean University of China,Qingdao 266003,China)

机构地区：[1]中国海洋大学海洋生命学院,山东青岛266003

出　　处：《海洋科学》2022年第2期46-54,共9页Marine Sciences

基　　金：山东省重点研发计划(2016GSF115042)。

摘　　要：为有效抑制萱藻种质保存和丝状体扩增时膨胀色球藻的污染,本实验应用实验生态学法,研究了恩诺沙星、磺胺嘧啶和呋喃西林3种抗菌药对共培养的萱藻丝状体和膨胀色球藻生长的影响。结果显示:在5~100 mg/L范围内,50 mg/L恩诺沙星适于除去萱藻种质保存或丝状体扩增过程中出现的膨胀色球藻,实验第18 d膨胀色球藻的日均增长率为-2.67%,萱藻丝状体的单室孢子囊发育良好;在10~150 mg/L范围内,100 mg/L磺胺嘧啶能有效抑制膨胀色球藻的生长,实验第18 d膨胀色球藻的日均增长率为-0.58%,萱藻丝状体日均增长率为2.91%,单室孢子囊发育良好;150 mg/L磺胺嘧啶会使萱藻丝状体的部分细胞呈浅褐色,单室孢子囊发育不良;在1~20 mg/L范围内,20 mg/L呋喃西林能抑制膨胀色球藻的生长,实验第18 d膨胀色球藻日均增长率为-0.78%,但同时影响了萱藻丝状体的正常生长,单室孢子囊发育不良。实验证明,50 mg/L恩诺沙星和100 mg/L磺胺嘧啶最适于解决萱藻种质保存和丝状体扩增过程中膨胀色球藻的污染。In order to inhibit the growth of Chroococcus turgidus during the germplasm preservation and the ampli-fication of filaments of Scytosiphon lomentaria,effects of enrofloxacin,sulfadiazine and nitrofurazone on the growth of filaments of S.lomentaria and C.turgidus in the co-cultured condition are studied by experimental ecol-ogy methods.Results indicated that:①In the range of 5~100mg/L,50mg/L enrofloxacin is suitable for inhibiting C.turgidus from the germplasm preservation and the amplification of filaments of S.lomentaria.The average daily growth rate of C.turgidus is–2.67%and the development of unilocular sporangia of filaments of S.lomentaria is well on the 18th day;②In the range of 10~150mg/L,100mg/L is the optimum concentration of sulfadiazine to in-hibit the growth of C.turgidus.On the 18th day,the average daily growth rate of C.turgidus is–0.58%,the average daily growth rate of S.lomentaria is 2.91%and the development of unilocular sporangia of filaments of S.lomentaria is well;The growth of filaments of S.lomentaria is in bad condition and unilocular sporangia was poorly developed at 150mg/L;③In the range of 1~20 mg/L,20 mg/L nitrofurazone has the inhibitory effect on C.turgidus and the filaments of S.lomentaria,the average daily growth rate of C.turgidus is–0.78%on the 18th day,and at the same time,the normal growth of filaments of S.lomentaria also be affect,and the unilocular sporangia developed poorly.All this experiments have proved that:50mg/L enrofloxacin and 100mg/L sulfadiazine are suit-able for solving the contamination of C.turgidus during the germplasm preservation and amplification of filaments of Scytosiphon lomentaria.

关 键 词：萱藻 丝状体 膨胀色球藻 共培养 抗菌药 

分 类 号：Q14[生物学—生态学]