短程反硝化除磷工艺运行及菌群结构研究  

Study on operation and bacterial community of short-cut denitrifying phosphorus removal process

作　　者：李微[1] 侯云鹤 王宇琦 王贺 陈一鸣 高明杰 祝雷[3] LI Wei;HOU Yunhe;WANG Yuqi;WANG He;CHEN Yiming;GAO Mingjie;ZHU Lei(School of Municipal and Environmental Engineering,Shenyang Jianzhu University,Shenyang Liaoning 110168;Liaoning Provincial Urban and Rural Construction Planning and Design Institute Co.,Ltd.,Shenyang Liaoning 110006;Shenyang Academy of Environmental Science,Shenyang Liaoning 110167)

机构地区：[1]沈阳建筑大学市政与环境工程学院,辽宁沈阳110168 [2]辽宁省城乡建设规划设计院有限责任公司,辽宁沈阳110006 [3]沈阳环境科学研究院,辽宁沈阳110167

出　　处：《环境污染与防治》2022年第3期285-291,共7页Environmental Pollution & Control

基　　金：辽宁省教育厅科研项目(No.lnqn202010);辽宁省科技厅自然科学基金资助项目(No.2019ZD0671)。

摘　　要：用间歇式活性污泥法快速富集短程反硝化聚磷菌(DPB),分析pH、污泥龄(SRT)、温度、碳源对系统脱氮除磷的影响,利用高通量测序技术研究污泥的菌群结构。试验结果表明:三阶段驯化法历经36 d成功富集DPB,pH为8.0,SRT为26 d,温度为24℃,COD为200.00 mg/L,乙酸钠作为碳源时,系统的反硝化除磷效果最佳,TP、亚硝酸盐氮去除率分别为93.22%、91.36%。具有除磷功能的Ignavibacterium和Povalibacter为系统的优势菌属。This study used batch activated sludge process to rapidly enrich short-cut denitrifying phosphorus removal bacteria(DPB)and analyzed the effect of pH,sludge retention time(SRT),temperature and carbon source on the removal of nitrogen and phosphorus in the system.Then,high throughput sequencing technology was used to study the bacterial community of sludge.The results showed that DPB were successfully enriched with three-phase acclimation method after 36 days,and the optimal treatment effect was achieved under the condition of pH 8.0,SRT 26 d,temperature 24℃,COD 200.00 mg/L and sodium acetate as carbon source,the removal rates of TP and nitrite nitrogen reached 93.22%,91.36%,respectively.Ignavibacterium and Povalibacter with phosphorus removal function were the dominant bacterial genus in the system.

关 键 词：短程反硝化聚磷菌 间歇式活性污泥法 菌群 

分 类 号：X703[环境科学与工程—环境工程]