大麦品种(系)HvPDIL5-1和HvEIF4E基因的单倍型及其黄花叶病抗性研究  

Haplotypes of HvPDIL5-1 and HvEIF4E and Resistance to Yellow Mosaic Disease of Different Barley Varieties(Lines)

作　　者：潘雨涵 洪益 徐肖 栾海业[2] 朱娟[1] 吕超[1] 郭宝健[1] 沈会权[2] 许如根[1] PAN Yuhan;HONG Yi;XU Xiao;LUAN Haiye;ZHU Juan;Lü Chao;GUO Baojian;SHEN Huiquan;XU Rugen(Key Lab of Plant Functional Genomics M.E/Jiangsu Key Lab of Crop Genomics Molecular Breeding/Jiangsu Co-Innovation Center for Modern Production Technology of Grain Crop/Yangzhou University Institutes of Agricultural Science and Technology Development,Yangzhou,Jiangsu 225009,China;Jiangsu Institute of coastal Agricultural Sciences,Yancheng,Jiangsu 224001,China)

机构地区：[1]植物功能基因组学教育部重点实验室/江苏省作物基因组学与分子育种重点实验室/江苏粮食作物现代产业技术协同创新中心/扬州大学农业科技发展研究院,江苏扬州225009 [2]江苏沿海地区农业科学研究所,江苏盐城224001

出　　处：《麦类作物学报》2022年第2期153-162,共10页Journal of Triticeae Crops

基　　金：国家重点研发计划项目(2018YFD1000703,2018YFD1000700);国家大麦青稞产业技术体系建设专项(CARS-05);江苏高校优势学科建设工程资助项目;江苏省农业科技自主创新项目[CX(19)3062]。

摘　　要：为了解大麦品种HvPDIL5-1和HvEIF4E基因的单倍型及其与大麦黄花叶病抗性的关系,以扬农啤系列、扬饲麦系列和其他来源的29个品种(系)为材料,在江苏扬州和盐城病圃进行大麦黄花叶病大田自然抗性鉴定;利用大麦HvPDIL5-1和HvEIF4E基因全CDS区的3对引物对供试品种(系)的HvPDIL5-1和HvEIF4E基因进行扩增、测序,分析两个黄花叶病抗性基因的单倍型及其抗性。结果表明,本试验年度两试点大麦黄花叶病发病程度较正常年份偏轻,29个品种(系)中有20个品种(系)在扬州和盐城对大麦黄花叶病同时表现为免疫或高抗,Ea52和苏B1403在扬州表现为免疫、在盐城表现为中抗,其余7个品种(系)在两试点均表现为中抗或中感。供试材料的基因存在4种单倍型,HvEIF4E基因存在14种单倍型。扬农啤2号、扬饲麦3号、扬农啤7号等10个品种(系)在扬州和盐城病圃对大麦黄花叶病均表现为免疫或高抗,但其HvPDIL5-1和HvEIF4E基因均为BaMMV-ASL感病单倍型,说明这些品种(系)携带有除rym1/11和rym4/5以外的其他黄花叶病抗性基因。携带抗性基因rym5的苏B1403在盐城病圃鉴定中表现为中抗,说明江苏大麦产区出现了拮抗rym5抗性基因的病毒株系,需进一步加强大麦黄花叶病抗性新基因的发掘。In order to understand the relationship between the haplotypes of barley genes HvPDIL5-1 and HvEIF4E and barley yellow mosaic disease resistance,in this study,29 varieties(lines)including Yangnongpi series,Yangsimai series and other sources,were used to identify the natural resistance to barley yellow mosaic disease in Yangzhou and Yancheng disease nurseries of Jiangsu Province.The HvPDIL5-1 and HvEIF4E genes of the tested varieties were amplified and sequenced using the primers of the whole CDS region of the HvPDIL5-1 gene and HvEIF4E gene,and the haplotypes of these two yellow mosaic disease resistance genes were analyzed.The results of resistance identification showed that the incidence of barley yellow mosaic disease in 2020 was lower than that in normal years.Among the test materials,20 varieties(lines)showed immunity or highly resistance in Yang-zhou and Yancheng;Ea52 and Su B1403 were immune in Yangzhou and moderately resistant in Yancheng,and the remaining seven varieties were moderately resistant or moderately susceptible to barley yellow mosaic disease.The test materials contain 4 haplotypes for HvPDIL5-1 gene and 14 haplotypes for HvEIF4E gene.10 varieties(lines)such as Yangnong 2,Yangsimai 3 and Yangnong 7 showed immunity or highly resistance to barley yellow mosaic disease in Yangzhou and Yancheng,but they all showed BaMMV-ASL susceptible haplotype in HvPDIL5-1 and HvEIF4E,indicating that these varieties(lines)may carry other yellow mosaic disease resistance genes except for rym1/11 and rym4/5.Su B1403 carrying the resistance gene rym5 in Yancheng disease nursery showed morderately resistanity,indicating that there may contain an antagonistic rym5 resistance gene virus strain in Jiangsu barley producing areas,and the excavation of new barley yellow mosaic disease resistance gene needs to be further strengthened.

关 键 词：大麦 黄花叶病 抗病基因 单倍型 亲缘关系 

分 类 号：S512.3[农业科学—作物学] S330