RP-HPLC法同时测定苓桂术甘汤中的甘草苷和原儿茶酸含量  

SIMULTANEOUS DETERMINATION OF GLYCYRRHIZIN AND PROTOCATECHUIC ACID IN GUIGUIZHUGAN DECOCTION BY RP-HPLC

作　　者：孟园[1] 王来兵 于姝燕 郭晓宇 王敏 于娟 李冰 刘涛 陈建平 MENG Yuan;WANG Laibing;YU Shuyan(Department of Pharmacy,Bayannur Hospital,Bayannur 015000 China)

机构地区：[1]巴彦淖尔市医院药剂科,内蒙古巴彦淖尔015000 [2]内蒙古医科大学药学院,010059

出　　处：《内蒙古医科大学学报》2021年第6期561-563,600,共4页Journal of Inner Mongolia Medical University

摘　　要：目的:建立RP-HPLC方法同时测定苓桂术甘汤中甘草苷和原儿茶酸的含量。方法:采用Eclipse Plus C18色谱柱,在260 nm波长检测,柱温25℃,流速1.0 mL·min^(-1),梯度洗脱,流动相为乙腈-0.1%磷酸水,进样量为10μL。结果:苓桂术甘汤中甘草苷和原儿茶酸的线性范围分别为2.12~21.15μg·mL^(-1)(r=0.9992)、1.36~13.60μg·mL^(-1)(r=0.9993);两成分的加样回收率的均值分别为98.37%(RSD=0.9%,n=6)、98.51%(RSD=1.1%,n=6);在此条件下,苓桂术甘汤中甘草苷和原儿茶酸的含量分别为618.6μg·g^(-1)、49.09μg·g^(-1),RSD均为0.9%。结论:所用方法简单灵敏、快速高效、易于操作,可用于测定苓桂术甘汤中甘草苷和原儿茶酸的含量。Objective:To establish a high performance liquid chromatography method for the simultaneous determination of glycyrrhizin and protocatechuic acid in Linggui Zhugan decoction.Methods:The chromatographic column was Eclipse Plus C18;detection at 260 nm wavelength;column temperature was 25℃;flow rate was 1.0 mL·min^(-1);gradient elution;the mobile phase was acetonitrile-0.1%phosphoricacid water;the injection volume is 10μL.Results:There was a good linear relationship between glycyrrhizin and protocatechuic acid in Linggui Zhugan Decoction in the range of2.12~21.15μg·mL^(-1),1.36~13.60μg·mL^(-1).The correlation coefficients were 0.9992 and 0.9993,respectively.The average recoveries of the two components were 98.37%(RSD=0.9%,n=6)and 98.51%(RSD=1.1%,n=6),respectively;Under these conditions,the contents of glycyrrhizin and protocatechuic acid in Lingguizhugan decoction were 618.6μg·g^(-1)and 49.09μg·g^(-1),respectively.The RSD is 0.9%.Conclusions:The method is sensitive,rapid,efficient and easy to operate.It can be used for the determination of glycyrrhizin and protocatechuic acid in Lingguizhugan Decoction.

关 键 词：RP-HPLC法 苓桂术甘汤 甘草苷 原儿茶酸 

分 类 号：R94[医药卫生—药剂学]