不同氮形态处理条件下杨树根尖差异表达基因的特征分析  

Characterization of Differentially Expressed Genes in Root Tips of Poplar Under Different Nitrogen Forms

作　　者：周婧[1] 李卓蓉 吴江婷 ZHOU Jing;LI Zhuo-rong;WU Jiang-ting(State Key Laboratory of Tree Genetics and Breeding,Key Laboratory of Silviculture of the National Forestry and Grassland Administration,Research Institute of Forestry,Chinese Academy of Forestry,Beijing,100091,China)

机构地区：[1]林木遗传育种国家重点实验室,国家林业和草原局森林培育重点实验室,中国林业科学研究院林业研究所,北京100091

出　　处：《林业科学研究》2022年第2期45-55,共11页Forest Research

基　　金：国家自然基金面上项目(32171739);国家自然基金青年项目(31500507);中国林科院基本科研业务费专项资金项目青年配套项目(CAFYBB2016QB005)。

摘　　要：[目的]利用高通量转录组测序技术,在硝态氮或铵态氮处理条件下,对杨树根尖差异表达基因进行了筛选和研究,同时分析和描述了差异表达基因对杨树根尖生长发育的影响,为后续开发高氮素吸收利用效率的杨树新种质提供科学依据。[方法]以灰杨幼苗根尖为材料,用0.5mmol·L^(-1)硝态氮(NO_(3)^(-))和0.5mmol·L^(-1)铵态氮(NH_(4)^(+))对幼苗处理10 d,并对植株根尖进行转录组测序以及生物信息学分析。[结果]硝态氮处理下的主根长度几乎是铵态氮处理条件下的一倍。从两种不同氮形态处理杨树根尖转录组文库中,筛选到2207个差异表达基因。通过差异基因GO和KEGG功能聚类分析,分别获得50个GO功能聚类和20条KEGG通路。进一步利用MapMan分析,筛选出36个氮代谢通路过程、各类氨基酸的生物合成以及代谢过程相关的差异表达基因。对这些差异基因互作调控网络分析发现,硝酸还原酶(Potri.005G172400)基因通过响应不同氮形态,在影响杨树根尖生长发育过程中发挥了重要作用。[结论]本研究获得了不同氮形态处理条件下杨树根尖差异表达基因,并对差异表达基因功能进行分析,有助于理解杨树通过响应不同氮形态影响根尖生长发育过程的分子机制。[Objective]To understand the molecular regulation mechanism of Populus×canescens root tips in response to different nitrogen forms.[Method]High-throughput transcriptome sequencing technology was used to screen and analyze the differentially expressed genes in poplar root tips under nitrate or ammonium treatments.At the same time,the effects of differently expressed genes on the growth and development process of poplar root tips were analyzed and described,which provides a scientific basis for the subsequent development of new poplar germplasm with high nitrogen absorption and utilization.The root tips of P.×canescens were treated with 0.5 mmol·L^(−1)nitrate(NO_(3)^(−))and 0.5 mmol·L^(−1)ammonium(NH_(4)^(+))for 10 days,and transcriptome sequencing and bioinformatics analysis were performed on the root tips of the plants.[Result]The root length under nitrate treatment was almost twice that under ammonium treatment.2207 differentially expressed genes were identified from the root tip transcriptome library of poplar treated with two nitrogen forms.Fifty GO functional clusters and 20 KEGG pathways were obtained by means of differential gene GO and KEGG functional clustering analysis,respectively.Furthermore,36 differentially expressed genes related to nitrogen metabolism pathway,biosynthesis of various amino acids and metabolic process were screened by MapMan analysis.Analysis of the regulatory network of these differential gene interactions showed that nitrate reductase(Potri.005G172400)gene played an important role in affecting root tip growth and development by responding to different nitrogen forms.[Conclusion]Differentially expressed genes of poplar root tips under different nitrogen form treatments were obtained.The functions of the differentially expressed genes were analyzed,which may help to understand the molecular mechanism of poplar root tip growth and development process by responding to different nitrogen forms.

关 键 词：转录组 差异表达基因 杨树根尖 硝态氮 铵态氮 

分 类 号：S718.46[农业科学—林学]