微小核糖核酸-205-5p靶向真核细胞翻译起始因子4E对人乳腺癌细胞MCF-7增殖、侵袭及上皮间质转化的影响  

Influence of miR-205-5p targeting eIF4E on proliferation,invasion and epithelial-mesenchymal transition of MCF-7 cells

作　　者：张娜贤 刘柳[1] 李刚 刘琳瑞 李元颖 ZHANG Naxian;LIU Liu;LI Gang;LIU Linrui;LI Yuanying(Department of Breast Cancer,Nanyang Second People's Hospital,Nanyang Henan 473000,China)

机构地区：[1]南阳市第二人民医院乳腺肿瘤科,河南南阳473000

出　　处：《中国药物警戒》2022年第3期259-264,共6页Chinese Journal of Pharmacovigilance

基　　金：河南省医学科技攻关计划项目(201801027)。

摘　　要：目的探讨微小核糖核酸-205-5p(miR-205-5p)靶向真核细胞翻译起始因子4E(eIF4E)对人乳腺癌细胞MCF-7增殖、侵袭及上皮间质转化的影响,为乳腺癌(MC)新型靶向治疗药物的研发提供参考。方法对MCF-7进行培养,miR-205-5p转染mimic-NC及miR-205-5p mimic以验证miR-205-5p与eIF4E靶向关系,同时将培养好的MCF-7细胞分为对照组(正常培养的MCF-7细胞)、mimic-NC组、pcDNA组、miR-205-5p mimic组、miR-205-5p组、eIF4E-pcDNA组、miR-205-5p+eIF4E-pcDNA组,分析miR-205-5p靶向eIF4E对MCF-7细胞增殖、迁移和上皮间质转化的影响。结果miR-205-5p mimic组的miR-205-5p相对表达量显著高于对照组和mimic-NC组,而eIF4E的mRNA表达水平则显著低于对照组和mimic-NC组(P<0.05),而eIF4E野生型、mimic均为阳性的Luciferase activity(R/F ratio)更低。eIF4E-pcDNA组eIF4E蛋白和mRNA相对表达量较对照组、pcDNA组明显高(P<0.05)。miR-205-5p组MCF-7细胞阳性率、菌落分布率和Ki67、PCNA蛋白表达水平较对照组、eIF4E-pcDNA组、miR-205-5p+eIF4E-pcDNA组明显低(P<0.05)。miR-205-5p组侵袭性细胞阳性率和侵袭性细胞数量较对照组、miR-205-5p+eIF4E-pcDNA组、eIF4E-pcDNA组明显低(P<0.05),而eIF4E-pcDNA组侵袭性细胞数量最高。miR-205-5p组MCF-7细胞上皮间质标志物E-Cadherin、N-Cadherin、Vimentin阳性表达率和蛋白表达水平明显低于对照组、miR-205-5p+eIF4E-pcDNA组、eIF4E-pcDNA组(P<0.05),而eIF4E-pcDNA组上皮间质标志物E-Cadherin、N-Cadherin、Vimentin阳性表达率和蛋白表达水平显著高于其他组(P<0.05)。结论miR-205-5p负靶向eIF4E能有效抑制MCF-7增殖、侵袭及上皮间质转化,有望为MC新型基因靶向治疗药物的研发提供重要参考。Objective To investigate the influence of micro ribonucleic acid-205-5p(miR-205-5p)targeting eukaryotic initiation factor 4E(eIF4E)on the proliferation,invasion and epithelial-mesenchymal transition of human breast cancer MCF-7 cells so as to provide reference for the development of new targeted therapeutic drugs against mammary cancer(MC).Methods MCF-7 cells were cultured,andm iR-205-5p was transfected intom imic-NC andm iR-205-5p m imic to verify the targeting relationship betweenm iR-205-5p ande IF4E.Meanwhile,the cultured MCF-7 cells were divided into the control group(normally cultured MCF-7 cells),mimic-NC group,pcDNA group,miR-205-5p mimic group,miR-205-5p group,e IF4E-pcDNA group,andm iR-205-5p+eIF4E-pcDNA group.The effect ofm iR-205-5p targeting eIF4E on the proliferation,migration and epithelial-mesenchymal transition of MCF-7 cells was analyzed.Results The relative expression level of miR-205-5p was significantly higher in the miR-205-5p mimic group than in the control group andm imic-NC group,while the mRNA expression level ofe IF4E was significantly lower(P<0.05).The luciferase activity(R/F ratio)of eIF4E wild-type and mimic positive group was lower.The relative expressions of eIF4E protein and mRNA were significantly higher in the eIF4E-pcDNA group than in the control group and pcDNA group(P<0.05).The positive rate of MCF-7 cells,colony distribution rate,K i67 andP CNA protein expression levels in them iR-205-5p group were significantly lower than those in the control group,eIF4E-pcDNA group,and miR-205-5p+eIF4E-pcDNA group(P<0.05).The positive rate and number of aggressive cells in the miR-205-5p group were significantly lower orsmaller than those in the control group,m iR-205-5p+eIF4E-pcDNA group,ande IF4E-pcDNA group(P<0.05),but the number of aggressive cells was the largest in eIF4E-pcDNA group.The positive expression rates and protein expression levels of the epithelial mesenchymal markersE-Cadherin,N-Cadherin,and Vimentin of MCF-7 cells were significantly lower in them iR-205-5p group than in

关 键 词：miR-205-5p EIF4E 人乳腺癌细胞 MCF-7 

分 类 号：R979.1[医药卫生—药品]