43%氟吡菌酰胺·肟菌酯悬浮剂的HPLC分析  

Analytical Method of Fluopyram · Trifloxystrobin 43% SC by HPLC

作　　者：王思威[1] 常虹 王潇楠 孙海滨[1] 刘艳萍[1] Wang Siwei;Chang Hong;Wang Xiaonan;Sun Haibin;Liu Yanping(Institute of Plant Protection,Guangdong Academy of Agricultural Sciences,Guangdong Provincial Key Laboratory of High Technology for Plant Protection,Guangzhou Guangdong 510640,China)

机构地区：[1]广东省农业科学院植物保护研究所,广东省植物保护新技术重点试验室,广东广州510640

出　　处：《农药科学与管理》2022年第1期36-42,共7页Pesticide Science and Administration

基　　金：广州市科技计划项目(202102080338);广东省乡村振兴战略专项项目(403-2018-XMZC-0002-90);国家荔枝龙眼产业技术体系项目(No.CARS-32-16);广东省农业科学院院长基金项目(202026)。

摘　　要：建立HPLC测定43%氟吡菌酰胺·肟菌酯悬浮剂中有效成分的分析方法。选用WondaSil C 18-WR反相色谱柱,以乙腈-水(体积比为60:40)为流动相,在波长220nm条件下测定试样中氟吡菌酰胺和肟菌酯的含量。结果表明:氟吡菌酰胺和肟菌酯的线性相关系数为0.9992,变异系数分别为0.29%和0.42%,平均回收率分别为100.28%和100.24%。该方法操作简单,分离效果好,精密度和准确度高,适用于复配制剂中氟吡菌酰胺和肟菌酯的定量分析。A HPLC method for simultaneous determination of the active ingredient contents in fluopyram·trifloxystrobin 43%SC was established,using WondaSil C 18-WR column(at 220nm)and the mixture of acetonitrile and water(60:40,v/v)as mobile phase.The linear correlation coefficients of 0.9992,the correlation of variation of 0.29%and 0.42%,and the average recoveries of 100.28%and 100.24%were obtained for fluopyram and trifloxystrobin,respectively.The method was demonstrated to be simple and convenient,with good separation,high precision and accuracy,and was suitable for quantitative analysis of fluopyram and trifloxystrobin in the formulation.

关 键 词：氟吡菌酰胺 肟菌酯 高效液相色谱 分析 

分 类 号：S482.2[农业科学—农药学] O657.72[农业科学—植物保护]