苜蓿根瘤菌接种数量与方式对接种效果的影响  

Effects of Rhizobial Population and Inoculation Method on the Efficiency of Alfalfa Rhizobium Inoculation

作　　者：李佳欢 希娜 漫静 任成[1] 邓波[1] LI Jia-huan;XI Na;MAN Jing;REN Cheng;DENG Bo(College of Grassland Science and Technology, China Agricultural University, Beijing 100193, China;Horticultural College, Shenyang Agricultural University, Shenyang, Liaoning Province 110866, China;School of Ecology and Environment, Inner Mongolia University, Hohhot, Inner Mongolia 010021, China;Institute of Botany, The Chinese Academy of Sciences, Beijing 100093, China)

机构地区：[1]中国农业大学草业科学与技术学院,北京100193 [2]沈阳农业大学园艺学院,沈阳辽宁110866 [3]内蒙古大学生态与环境学院,呼和浩特内蒙古010021 [4]中国科学院植物研究所,北京100093

出　　处：《草地学报》2022年第3期743-749,共7页Acta Agrestia Sinica

基　　金：自治区区域协同创新专项(科技援疆计划)(2019E0211)资助。

摘　　要：本研究以‘中苜1号’苜蓿和gfp-Sm1021根瘤菌为试验材料,设置3个接种量和2种接种方式,通过温室试验探究接种量和接种方式对苜蓿根瘤菌结瘤固氮的影响。结果表明:提高接种量能增加苜蓿结瘤数、地上生物量和蛋白含量,降低地下生物量。接种量增加到(1.9×10^(5))个·株^(-1)后,种子接种处理中,继续增加接种量不能提高结瘤数;沟施接种处理中,继续增加接种量能增加结瘤数但降低地上生物量。相同接种量下,沟施可提高苜蓿的结瘤数,增加的根瘤主要位于0~4 cm侧根、4~8 cm主根、4~8 cm侧根和8~12 cm侧根。0~4 cm主根、0~4 cm侧根和4~8 cm主根的根瘤固氮酶活性显著高于其它部位。综上,接种量较低时,沟施接种优于种子接种,接种量较高时,结合根瘤的分布特征和固氮酶活性,沟施接种处理中结瘤数增加带来的固氮优势下降。We assessed the effects of inoculant population and rhizobial position on rhizobial inoculation efficiency in the greenhouse using alfalfa(Medicago sativa L.‘Zhongmu No.1’)and rhizobial strain gfp-Sm1021.The results showed that an increased inoculant population in a certain range increased nodules,as well as aboveground biomass and protein content,but decreased belowground biomass.For seed inoculation,nodule number and aboveground biomass could not be increased by redundancy rhizobia when the inoculant population reached 1.9×10^(5)per·plant^(-1).For in-furrow inoculation,a higher inoculant population increased nodule number while decreased aboveground biomass of alfalfa.In-furrow inoculation produced more nodules,relative to seed inoculation,and the most redundant nodules were located on 0~4 cm lateral root,4~8 cm tap root,4~8 cm lateral root,and 8~12 cm lateral root.The nitrogen-fixing ability of nodules located on 0~4 cm taproot,0~4 cm lateral root,and 4~8 cm taproot was significantly higher than others.In conclusion,in-furrow inoculation was more effective than seed inoculation when the inoculant population was small.However,when the inoculum amount was high,combined with the distribution characteristics of nodules on the root system and the nitrogenase activity of nodules at different root positions,the increase of nodules in in-furrow inoculation cannot bring the desired benefits.

关 键 词：根瘤菌接种量 接种方式 根瘤菌定殖量 结瘤数 根瘤分布 固氮酶活性 

分 类 号：S551.7[农业科学—作物学]