甘加藏羊发情周期和乏情期HPO轴中THRβmRNA和蛋白的表达分布  

Expression and distribution of THRβ mRNA and protein in HPO axis of Ganja Tibetan sheep during estrus cycle and anestrus

作　　者：刘莉莉 高何璇 杨亚文 包莹莹 何玉琴[1] 杨志杰[1] 陈卫刚 葛文博 LIU Lili;GAO Hexuan;YANG Yawen;BAO Yingying;HE Yuqin;YANG Zhijie;CHEN Weigang;GE Wenbo(College of Life Science and Technology, Gansu Agricultural University, Lanzhou 730070, China;Network Finance Department of Lanzhou Bank, Lanzhou 730070, China;College of Veterinary Medicine, Gansu Agricultural University, Lanzhou 730070, China)

机构地区：[1]甘肃农业大学生命科学技术学院,甘肃兰州730070 [2]兰州银行网络金融部,甘肃兰州730070 [3]甘肃农业大学动物医学院,甘肃兰州730070

出　　处：《浙江农业学报》2022年第3期480-488,共9页Acta Agriculturae Zhejiangensis

基　　金：国家自然科学基金(31960689)。

摘　　要：甲状腺激素(TH)通过与其靶细胞受体特异性结合发挥其生物学效能。为阐明松果体、下丘脑-垂体-卵巢轴(Hypothalamic-pituitary-ovary axis,HPOA)甲状腺激素受体β(Thyroid hormone receptorβ,THRβ)基因和蛋白的表达及分布对季节性繁殖动物甘加藏羊(Ovis aries)生殖活动的调节。本研究以处于发情周期和乏情期的甘加藏羊为研究对象,应用RT-qPCR、Western blot和免疫组织化学方法检测和分析了松果体、HPO轴中THRβmRNA及其蛋白的表达和分布。结果显示,THRβmRNA及其蛋白在松果体和HPO轴组织中均有表达和分布。在松果体中,THRβmRNA及其蛋白的表达量均在乏情期最高,显著(P<0.05)高于发情周期;在下丘脑中,THRβmRNA表达量在发情后期显著(P<0.05)高于其他时期,蛋白表达量在间情期显著(P<0.05)高于其他时期;在垂体中,THRβmRNA及其蛋白的表达量在间情期显著(P<0.05)高于其他时期;在卵巢中,THRβmRNA及其蛋白的表达量在发情后期显著(P<0.05)高于其他时期。免疫组织化学结果显示:THRβ免疫阳性产物主要分布在松果体细胞的细胞浆,下丘脑的神经胶质细胞、大神经元胞体和椎体细胞胞浆,腺垂体的嗜酸性和嗜碱性细胞胞浆,卵巢的颗粒细胞胞浆、卵泡内膜细胞及黄体细胞。甘加藏羊发情周期和乏情期松果体、HPOA组织中THRβmRNA和蛋白的差异性表达,表明THRβ参与调控甘加藏羊的生殖活动。Thyroid hormone(TH)exerted its biological effects by specifically binding to its target cell receptor.In order to clarify the regulation of thyroid hormone receptorβ(THRβ)gene and its protein in the pineal gland and Hypothalamic-pituitary-ovary(HPO)axis of the reproductive activity of seasonal breeding animals,Ganjia Tibetan sheep(Ovis aries).In this study,the healthy Ganjia Tibetan sheep during the estrus cycle and anestrus were used as the research object.RT-qPCR,Western blot and immunohistochemical staining were used to detect and analyze the expression and distribution of THRβmRNA and its protein in the pineal gland and HPOA.The results showed that THRβmRNA and its protein were expressed and distributed in the pineal gland and HPOA tissues.The expression of THRβmRNA and its protein in the pineal gland in the anestrus were significantly(P<0.05)higher than that in the estrus cycle.In hypothalamus,the expression of THRβmRNA in the metestrus were significantly(P<0.05)higher than that in other stages while the expression of its protein in the disestrus were significantly(P<0.05)higher than that in other stages;In pituitary gland,the expression of THRβmRNA and its protein in the diestrus were significantly(P<0.05)higher than that in other stages.In ovary,the expression of THRβmRNA and its protein in the metestrus were significantly(P<0.05)higher than that in other stages.The immunohistochemical results showed that THRβwas mainly distributed in the eosinophilic cytoplasm of the pineal,glial cells,large neuron cell bodies and vertebral cell cytoplasm of the hypothalamic,eosinophilic and basophilic cytoplasm of adenohypophysis,granulosa cell cytoplasm,follicular inner membrane cells and luteal cells of the ovary.THRβmRNA and its protein in the pineal gland and HPOA showed different changes during the estrus and anestrus,which indicated that THRβgene and its protein were involved in the regulation of the reproductive activities of the Ganjia Tibetan sheep.

关 键 词：甘加藏羊 下丘脑-垂体-卵巢轴 发情周期 乏情期 

分 类 号：S826[农业科学—畜牧学]