西洋参细胞悬浮培养体系的建立  

Research on Rapid Propagation of Panax Quinquefolium Callus and Establishment of Cell Suspension Culture System

作　　者：庞富洲 韦坤华 王硕[4] 邱莉[1] 梁莹[2,3] 韦范[2,3] 秦双双[2,3] 强宝宝[2,3] 缪剑华 PANG Fuzhou;WEi Kunhua;WANG Shuo;QIU Li;LIANG Ying;WEI Fan;QIN Shuangshuang;QIANG Baobao;MIAO Jianhua(Pharmaceutical College,Guangxi Medical University Nanning,Guangxi 530021;Guangxi Key Laboratory of Medicinal Resources Protection and Genetic Improvement,Guangxi Botanical Garden of Medicinal Plant Nanning,Guangxi 530023;Guangxi Engineering Research Center of TCM Resource Intelligent Creation,Guangxi Botanical Garden of Medicinal Plants Nanning,Guangxi 530023;Guangxi Zhuang Autonomous Region Traditional Chinese Medicine Resources Intelligent Creation Engineering Research Center Nanning,Guangxi 530023)

机构地区：[1]广西医科大学药学院,广西南宁530021 [2]广西药用植物园广西药用资源保护与遗传改良重点实验室,广西南宁530023 [3]广西药用植物园广西壮族自治区中药资源智慧创制工程研究中心,广西南宁530023 [4]广西药用植物园西南濒危药材资源开发国家工程实验室,广西南宁530023

出　　处：《现代农业研究》2022年第3期127-132,共6页Modern Agriculture Research

基　　金：广西创新驱动发展专项(桂科AA18242040);对发展中国家科技援助项目(KY201904001);中央引导地方科技发展专项资金项目(桂科ZY20198018)。

摘　　要：目的:建立西洋参细胞悬浮培养体系。方法:通过不同激素和物理影响因素筛选出西洋参细胞培养的最佳条件。结果:将西洋参愈伤组织接种于附加0.5mg·L^(-1)2,4-D+0.1 mg·L^(-1)KT+0.2 mg·L^(-1)NAA的MS培养基上最适合西洋参愈伤组织的生长;用西洋参愈伤组织制作西洋参细胞,发现西洋参悬浮细胞生长曲线类似“S”型;通过改变西洋参细胞悬浮培养的外界条件发现,西洋参悬浮细胞生长的最佳接种量为1.5 g/30mL,摇床转速和光照条件对西洋参细胞生长影响没有显著差异。结论:建立了西洋参细胞悬浮培养体系,该技术为西洋参细胞的扩大培养和有效成分的提取提供了物质基础。Establishing a suspension culture system of American ginseng cells.Methods:The best conditions of cell culture of Panax quinquefolium were screened out by different hormones and physical factors Results:The callus of Panax quinquefolium was inoculated with 0.5 mg·L^(-1)2,4-D+0.1 mg·L^(-1)KT+0.2 mg·L^(-1)NAA was the most suitable medium for the growth of Panax quinquefolium callus;using Panax quinquefolium callus to make Panax quinquefolium suspension cells,it was found that the growth curve of Panax quinquefolium suspension cells was similar to"s"type;by changing the external conditions of Panax quinquefolium cell suspension culture,it was found that the best inoculation amount for the growth of Panax quinquefolium suspension cells was 1.5 g/30mL.The growth of Panax quinquefolium cells was not statistically significant by light and the rotation speed of shaking table.Conclusion:The suspension culture system of Panax quinquefolium cells was established,which provided material basis for the expansion of cell culture and the extraction of effective components.

关 键 词：西洋参 愈伤组织 悬浮培养 增殖倍数 正交试验 

分 类 号：Q813.11[生物学—生物工程] A[哲学宗教—马克思主义哲学]