QuEChERS/LC-MS/MS法测定人参中30种农药残留  

Determination of 30 Pesticide Residues in Ginseng Using QuEChERS/LC-MS/MS

作　　者：毕融冰 王玉方[1] 罗婧[1] 赵卉[1] BI Rong-bing;WANG Yu-fang;LUO Jing;ZHAO Hui(Institute of Special Animal and Plant Sciences of Chinese Academy of Agricultural Sciences,Changchun 130000,Jilin,China)

机构地区：[1]中国农业科学院特产研究所,吉林长春130000

出　　处：《食品研究与开发》2022年第6期157-166,共10页Food Research and Development

摘　　要：建立人参中30种农药残留的快速样品处理,联合液相色谱-串联质谱(quick,easy,cheap,effective,rugged and safe/liquid chromatography tandem mass spectrometry,QuEChERS/LC-MS/MS)检测方法。通过对快速样品处理法(QuEChERS)的净化剂配比和LC-MS/MS质谱条件等优化,探究人参中30种农药多残留的最优测定方法。确定C18(100 mg)为净化剂,无水硫酸钠为提取盐包,30种农药多残留的回收率范围均在60%~120%,线性关系良好,相关系数均不低于0.99,变异系数范围均小于15%,测定低限均不大于2020版《中国药典》规定的定量限。该方法适用于人参中30种农药多残留的测定。A method for the determination of 30 pesticide residues in ginseng by using a quick,easy,cheap,effective,rugged and safe liquid chromatography tandem mass spectrometry(QuEChERS/LC-MS/MS)was established.The ultimate protocol was developed upon optimization of the QuEChERS purification agent ratio and mass spectrometry conditions.C18(100 mg)and anhydrous sodium sulfate were used as the purifying agent and extraction salt,respectively.The recovery rate of 30 types of pesticide residues was in the range of 60%-120%.Moreover,the final products showed a significant linear relationship with a correlation coefficient≥0.99 and a variation coefficient<15%.The lower limit of detection was similar to the quantitation reported in the《Chinese Pharmacopoeia》(2020).The finding suggests that the optimized method is suitable for the determination of 30 pesticide residues in ginseng.

关 键 词：人参 农药多残留 液相色谱-串联质谱法(LC-MS/MS) QuEChERS法 方法学考察 

分 类 号：O657.63[理学—分析化学] R284.1[理学—化学]