基于金纳米粒子团聚机理快速比色测定甲基硫菌灵  

Rapid colorimetric detection of thiophanate-methyl based on the agglomeration mechanism of gold nanoparticles

作　　者：覃国新 李慧玲 闫飞燕 何洁 韦宇宁 王海军 王静[2] 金茂俊[2] 劳水兵 杨玉霞 莫仁甫 罗丽红 陈泳锨 周其峰 QIN Guo-xin;LI Hui-ling;YAN Fei-yan;HE Jie;WEI Yu-ning;WANG Hai-jun;WANG Jing;JIN Mao-jun;LAO Shui-bing;YANG Yu-xia;MO Ren-fu;LUO Li-hong;CHEN Yong-xian;ZHOU Qi-feng(Institute for Agricultural Product Quality Safety and Testing Technology,Guangxi Academy of Agricultural Sciences,Quality Inspection and Test Center for Sugarcane and Its Product,China Ministry of Agriculture(Nanning),Nanning 530007,China;Key Laboratory for Agro-Product Quality and Safety,Ministry of Agriculture,Institute of Quality Standard and Testing Technology for Agro-Products,Chinese Academy of Agricultural Sciences,Beijing 100081,China)

机构地区：[1]广西壮族自治区农业科学院农产品质量安全与检测技术研究所,农业部甘蔗品质监督检验测试中心(南宁),南宁530007 [2]中国农业科学院农业质量标准与检测技术研究所,农业部农产品质量安全重点实验室,北京100081

出　　处：《农药》2022年第3期184-188,191,共6页Agrochemicals

基　　金：广西自然科学基金面上项目(2020GXNSFAA159073);广西科技重大专项(桂科AA20302016-6);科技先锋队‘强农富民’‘六个一’专项行动(桂农科盟202116);广西农业科学院基本科研业务专项资助项目(桂农科2021YT139,桂农科2021YT138);广西农业科学院科技发展基金项目(桂农科2021JM119)。

摘　　要：[目的]基于金纳米粒子(gold nanoparticle,AuNPs)的团聚机理,建立一种直接、快速、可视化的甲基硫菌灵农药检测方法。[方法]在酸性条件下,甲基硫菌灵分子上的=O键和=S键质子化,形成-OH和-SH,甲基硫菌灵通过Au-S键连接到AuNPs表面,再通过分子间的氢键相互作用,使甲基硫菌灵分子间相互连接,从而引起AuNPs的团聚,溶液颜色由酒红色变成蓝黑色,AuNPs在525 nm处的吸光度强度相应减弱,通过比色分析法可以实现对甲基硫菌灵的检测。[结果]甲基硫菌灵在0.05~5 mg/L范围内与AuNPs的相对吸光强度呈现良好的线性关系,相关系数R2为0.9928,检测限为0.04 mg/L。在番茄样本中的加标回收率在82.0%~93.4%之间。[结论]该方法能满足蔬菜实际样品中甲基硫菌灵残留快速检测的需求。[Aims]This study aims to develop a directly fast and visual approach for colorimetric detection of thiophanate-methyl pesticide based on the agglomeration mechanism of gold nanoparticles(AuNPs). [Methods]After protonation of thiophanate-methyl at the acidic conditions, the thiophanate-methyl can be connected to the surface of AuNPs through Au-S bonds. The thiophanate-methyl are interconnected by hydrogen bond. So that the AuNPs can aggregate. The color of the solution changed from wine red to blue black. And the absorbance intensity of AuNPs also weakened correspondingly. [Results]So an inverse relationship between the relative correlation intensity of AuNPs and thiophanate-methyl concentration in the range from 0.05 to 5 mg/L was found. The correlation coefficients and sensitivity of assay were 0.9928 and 0.04 mg/L, respectively. The recoveries from tomato samples spiked with different concentrations of thiophanate-methyl were in the range of 82.0-93.4%. [Conclusions]This assay can meet the requirement well of rapid detection of thiophanate-methyl residue in vegetable samples.

关 键 词：纳米金 甲基硫菌灵农药检测 比色分析法 

分 类 号：TQ450.7[化学工程—农药化工]