8.2%萎锈灵·咯菌腈包衣对棉花幼苗耐寒性的生理特征的影响  

Effects of 8.2% carboxin-fludioxonil from seed coating formulation on physiological characteristics of cold tolerance of cotton

作　　者：雷斌[1,2] 周小云 刘梦丽[3] 李进 卢彬 张军高[1,5] 李杰 袁豆豆 梁晶 LEI Bin;ZHOU Xiao-yun;LIU Meng-li;LI Jin;LU Bin;ZHANG Jun-gao;LI Jie;YUAN Dou-dou;LIANG Jing(Research Institute of Nuclear Technology and Biotechnology,Xinjiang Academy of Agricultural Sciences/Key Laboratory of Oasis-Desert Crop Physiology Ecology and Cultivation of Ministry of Agricultural and Rural Affairs,Urumqi 830091,China;Xinjiang Research Center of Engineering and Technology for Crop Chemical Regulation,Urumqi 830091,China;Xinjiang Plant Protection Station,Urumqi 830049,China;Xinjiang Academy of Analysis and Testing,Urumqi 830011,China;Xinjiang Oasis Xingyuan Agricultural Science and Technology Limited Liability Company,Urumqi 830091,China)

机构地区：[1]新疆农业科学院核技术生物技术研究所农业农村部荒漠绿洲作物生理生态与耕作重点实验室,乌鲁木齐830091 [2]新疆作物化学调控工程技术研究中心,乌鲁木齐830091 [3]新疆维吾尔自治区植保站,乌鲁木齐830049 [4]新疆维吾尔自治区分析测试研究院,乌鲁木齐830011 [5]新疆绿洲兴源农业科技有限责任公司,乌鲁木齐830091

出　　处：《农药》2022年第3期230-234,共5页Agrochemicals

基　　金：国家自然科学基金(31760073);国家自然科学基金(32060429);天山创新团队计划(2020D1400);天山雪松计划(2020XS16)。

摘　　要：[目的]为研发提高棉花幼苗耐寒能力的技术及产品,通过萎锈灵·咯菌腈复配进行种子处理,验证其提高棉种耐低温冷害能力功效,为该制剂的开发及应用提供理论基础。[方法]以对温度敏感的"新陆早57"棉种为实验材料,以基础种衣剂包衣处理为对照,将咯菌腈和萎锈灵添加到基础种衣剂中制成1%咯菌腈、7.2%萎锈灵、8.2%萎锈灵·咯菌腈的种衣剂分别进行包衣,采用人工模拟低温处理,4℃低温胁迫3 d后观测各包衣处理对低温条件下棉花幼苗生长情况和耐寒性的生理响应。[结果]萎锈灵·咯菌腈复配包衣处理棉花幼苗相关指标差异最大,与对照比较,相对电导率降低8.49%,丙二醛含量降低8.08%,可溶性糖的含量提高7.41%,超氧化物歧化酶酶的活性增强8.56%;脱落酸含量增加7.84%,冷害指数下降5.72%,且差异均显著。相关性分析表明:棉苗冷害指数与相对电导率、超氧化物歧化酶、脱落酸、赤霉素等相关指标有显著相关性。[结论]在低温胁迫下,萎锈灵·咯菌腈复配组合较单个杀菌剂更能诱导细胞内部保持较高的保护酶活性、较快清除植物体内活性氧自由基、加快丙二醛及脯氨酸含量的积累,保持较高的脱落酸含量和较低的吲哚乙酸、赤霉素含量,从而提高棉花幼苗抵御冷害能力。8.2%萎锈灵·咯菌腈种衣剂已表现出增强棉苗耐寒功能,可进行开发、推广。[Aims]T his s tudy aims to develop the technology and products to improve the cold tolerance of cotton seedlings, the effect of improving the cold tolerance of cotton seeds was verified through carboxin and fludioxonil mixture seed coating, so as to provide a theoretical basis for the development and application of the preparation.[Methods]With the temperature sensitive "Xinluzao 57" cotton seed as the experimental material and the coating treatment of the basic seed coating agent as the control, fludioxonil and carboxin were added to the basic seed coating agent to make 1% fludioxonil, 7.2% carboxin, and 8.2% carboxin-fludioxonil respectively for coating. The artificial low temperature treatment was used. After 3 days of low temperature stress at 4 ℃, the physiological responses of each coating treatment to the growth and cold tolerance of cotton seedlings under low temperature were observed. [Results]The results showed that the difference of related indexes of cotton seedlings treated with carboxin fludioxonil mixture coating was the largest. compared with the control, the relative conductivity(REC) decreased by 8.49%,malondialdehyde(MDA) content decreased by 8.08%, soluble sugar(SS) content increased by 7.41%, and superoxide dismutase(SOD) enzyme activity increased by 8.56%;abscisic acid(ABA)content increased by 7.84% and chilling injury index(CI) decreased by 5.72%. The correlation analysis showed that the chilling injury index of cotton seedlings was significantly correlated with REC, SOD, ABA and gibberellin(GA). [Conclusions]Under low temperature stress,compared with a single fungicide, coating cotton seeds with carboxin·fludioxonil formulation can induce higher protective enzyme activity in cells, faster scavenge reactive oxygen free radicals in plants, accelerate the accumulation of malondialdehyde and proline, maintain higher ABA content and lower Indoleacetic acid(IAA) and GA content, so as to improve the ability of cotton seedlings to resist cold injury. Therefore, the coating cotton seeds with 8.2

关 键 词：萎锈灵 咯菌腈 棉花 低温胁迫 幼苗耐寒性 

分 类 号：S482.8[农业科学—农药学]