Anionic surfactant sulfate dodecyl sodium (SDS)-induced thermodynamics and conformational changes of collagen by ultrasensitive microcalorimetry  

作　　者：Jie Zhang Chunhua Wang Fengteng Zhang Wei Lin 

机构地区：[1]Department of Biomass and Leather Engineering,Key Laboratory of Leather Chemistry and Engineering of Ministry of Education,Sichuan University,Chengdu 610065,China [2]National Engineering Research Center of Clean Technology in Leather Industry,Sichuan University,Chengdu 610065,China

出　　处：《Journal of Leather Science and Engineering》2021年第1期224-229,共6页皮革科学与工程（英文）

基　　金：The financial support of National Natural Science Foundation(NNSF)of China(21978177);the National Key R&D Program of China(2019YFC1904500);International Science and Technology Innovation Cooperation project of Sichuan Province(2020YFH0081)is gratefully acknowledged.

摘　　要：In this communication,sulfate dodecyl sodium(SDS)-induced thermodynamics and conformational changes of collagen were studied.We used ultrasensitive differential scanning calorimetry(US-DSC)to directly monitor the thermal transition of collagen in the presence of SDS.The results show that SDS affects the conformation and thermal stability of collagen very differently depending on its concentrations.At C SDS≤0.05mM,the enhanced thermal stability of collagen indicates the stabilizing effect by SDS.However,a further increase of SDS leads to the denaturation of collagen,verifying the well-known ability of SDS to unfold proteins.This striking difference in thermodynamics and conformational changes of collagen caused by SDS concentrations can be explained in terms of their interactions.With increasing SDS,the binding of SDS to collagen can be dominated by electrostatic interaction shifting to hydrophobic interaction,and the latter plays a key role in loosening and unfolding the triple-helix structure of collagen.The important finding in the present study is the stabilizing effect of SDS on collagen molecules at extreme low concentration.

关 键 词：COLLAGEN Sodium dodecyl sulfate Conformational change MICROCALORIMETRY 

分 类 号：R47[医药卫生—护理学]