萆薢降酸方对高尿酸血症小鼠模型的药效作用及其对肾脏尿酸盐转运体表达的影响  

Effect of Bixie deacidification fang on hyperuricemia mouse model and its effect on the expression of renal urate transporter

作　　者：王琼[1] 周冰[1] 杨全伟[2] WANG Qiong;ZHOU Bing;YANG Quanwei(Wuhan Hospital of Traditional Chinese Medicine,Wuhan 430000,China;Wuhan No.1 Hospital,Wuhan 430000,China)

机构地区：[1]武汉市中医医院,湖北武汉430000 [2]武汉市第一医院,湖北武汉430000

出　　处：《药学实践杂志》2022年第2期143-145,共3页Journal of Pharmaceutical Practice

基　　金：武汉市卫生健康委科研项目(WZ21A01、WZ18D12)。

摘　　要：目的研究萆薢降酸方提取物对高尿酸血症小鼠的抗高尿酸血症药效作用及其对肾脏蛋白的转运机制。方法采用氧嗪酸钾致高尿酸血症小鼠模型,观察萆薢降酸方提取物对高尿酸血症小鼠的影响。以220、440、880mg/kg的剂量,连续10 d,以别嘌醇(5mg/kg)为阳性对照。采用比色法测定血清、尿酸、肌酐水平。同时用Western blot法分析肾脏尿酸盐转运蛋白1(URAT1)和阴离子转运蛋白1(OAT1)的蛋白质水平。结果与模型组比较,高剂量萆薢降酸方可抑制血清中黄嘌呤氧化酶(XOD)活性(18.12±1.33u/L)和肝脏活性蛋白(70.15±5.20u/g)(P<0.05),降低血清尿酸(2.04±0.64mg/L)(P<0.05)和血清肌酐(0.35±0.18mol/L)尿素氮(8.83±0.71mmol/L)(P<0.05);升高尿酸(38.34±8.23mg/L)和尿肌酐(34.38±1.98mmol/L)水平,URAT1表达水平下调,OAT1表达水平上调(P<0.05)。结论萆薢降酸方可能通过上调OAT1蛋白表达促进尿酸排泄、下调URAT1蛋白表达抑制尿酸重吸收的双重调节功能来促进尿酸在肾脏中的排泄。Objective To investigate the anti-hyperuricemia effects of Bixie deacidification fang on hyperuricemia mice and its mechanism of renal protein transport.Methods The effects of Bixie deacidification fang were investigated on hyperuricemia mice induced by potassium oxonate.Bixie deacidification fang was administered to hyperuricemia mice daily at doses of 220,440 and 880 mg/kg for 10 days,and allopurinol(5mg/kg)was given as positive control.Serum and urine levels of uric acid and creatinine were determined by colorimetric method.Simultaneously,protein levels of urate transporter 1(URAT1)and organic anion transporter 1(OAT1)in the kidney were analyzed by Western blot.Results Compared with the model group,highdose of Bixie deacidification fang inhibited xanthine oxidase(XOD)activities in serum(18.12±1.33 u/L)and that in liver(70.15±5.20 u/g protein)(P<0.05),decrease levels of serum uric acid(2.04±0.64mg/L)(P<0.05)and serum creatinine(0.35±0.18μmol/L)and blood urea nitrogen(BUN)(8.83±0.71mmol/L)(P<0.05),ncreased levels of urine uric acid(38.34±8.23mg/L),urine creatinine(34.38±1.98mmol/L),down-regulated of URAT1 and up-regulated of OAT1 protein expressions(P<0.05)in the renal tissue of hyperuricemia mice.Conclusion Bixie deacidification fang recipe may promote the excretion of uric acid in the kidney by up-regulating the expression of OAT1 protein to promote the excretion of uric acid,and down-regulating the expression of URAT1 protein to inhibit the reabsorption of uric acid.

关 键 词：萆薢降酸方提取物 高尿酸血症 肾脏尿酸转运蛋白 尿酸 肌酐 

分 类 号：R285[医药卫生—中药学]