2种冷冻时长进行液氮冷冻法犬股骨头坏死造模的效果比较  

A comparative analysis of canine femur head necrosis models induced by liquid nitrogen freezing with two freezing durations

作　　者：楼鹏强 邓小磊[1] 魏波 汤朔 侯德才[1] LOU Pengqiang;DENG Xiaolei;WEI Bo;TANG Shuo;HOU Decai(The Affliated Hospital of Liaoning University of Traditional Chinese Medicine,Shenyang 110032,Liaoning,China)

机构地区：[1]辽宁中医药大学附属医院,辽宁沈阳110032

出　　处：《中医正骨》2022年第3期3-7,31,共6页The Journal of Traditional Chinese Orthopedics and Traumatology

基　　金：辽宁省科学技术计划项目(2017225043)。

摘　　要：目的:比较2种冷冻时长进行液氮冷冻法犬股骨头坏死造模的效果。方法:将12只SPF级健康成年雄性比格犬随机分为2组,每组6只。均采用液氮冷冻法进行股骨头坏死造模,液氮冷冻时长分别为8min(冷冻8min组)和4min(冷冻4min组)。造模手术后观察并记录动物的一般情况。造模后9周,进行X线和MRI检查,并以MRI影像作为判断股骨头坏死的标准。影像学检查结束后,处死动物,取出造模侧股骨头,进行大体观察和病理学检查(HE染色)。结果:①一般情况。至最终处死前,2组均无动物死亡。造模手术后2d内,所有动物均开始出现饮食减少、精神萎靡、活动减少等表现。冷冻4min组动物的上述表现均在1周内改善;冷冻8min组动物的上述表现约在2周后逐步恢复正常,切口愈合缓慢,而且均出现了跛行现象。冷冻8min组的1只动物在造模手术后第3天出现术区皮肤红肿,造模手术后1周时出现对应部位皮肤组织坏死及化脓,造模手术后4周时愈合。②影像学检查结果。X线片显示,冷冻8min组动物造模侧股骨头均出现了不同程度的变化,如骨质硬化、关节间隙变窄,但变化不明显,且部分动物的股骨头出现了囊性变;冷冻4min组仅2只动物的股骨头出现了轻微的骨质硬化。MRI显示,冷冻8min组所有动物T2WI上均可见股骨头骨髓水肿和关节腔积液表现,T1WI上均可见线样低信号,均达到股骨头坏死标准;冷冻4min组4只动物T2WI上可见股骨头骨髓水肿和关节腔积液表现,其余2只动物的T2WI上仅可见关节腔积液表现,T1WI上均无明显改变。③大体观察结果。冷冻8min组股骨头扁平、表面较粗糙,部分股骨头出现软骨裂纹,部分股骨头软骨磨损严重;冷冻4min组股骨头形态无明显改变,表面较光滑,仅部分动物的股骨头出现了软骨裂纹或轻微软骨磨损。④病理学检查结果。冷冻8min组股骨头组织骨陷窝内大部分骨细胞核消失,同时�Objective:To compare the effects of canine femur head necrosis(FHN)models induced by liquid nitrogen freezing(LNF)with two freezing durations.Methods:Twelve SPF-grade healthy adult male beagle canines were selected and randomly assigned to 2 groups,6 ones in each group,and they were subjected to LNF for 8 minutes(8-min freezing group)and 4 minutes(4-min freezing group)respectively for inducing FHN.The general conditions of the model canines were then observed and recorded after the modeling surgery.At 9 weeks after the modeling,all model canines underwent X-ray examination and magnetic resonance imaging(MRI)examination and the MRI images were used as the standard for confirming whether the FHN models were built successfully.After the end of imaging examina-tion,the model canines were sacrificed and their femur heads were harvested from the modeling side for gross observation and pathological examination(HE staining).Results:①No model canine died in the 2 groups before the final execution.Within 2 days after the modeling surgery,all model canines began to exhibit such symptoms as decreased food intake,mental fatigue and reduced movement,which was im-proved within 1 week in4-min freezing group,and gradually returned to normal about 2 weeks later in 8-min freezing group with the symptoms of slow wound healing and claudication.One model canine in 8-min freezing group exhibited the symptoms as skin redness and swelling on the 3rd day after the modeling surgery,skin tissue necrosis and pyosis at the corresponding site at 1 week after the modeling sur-gery,and healed at 4 weeks after the modeling surgery.②The results of X-ray examination showed that the unremarkable changes in different degrees,manifesting as osteosclerosis and joint space narrowing,were observed in femur heads at the modeling side of model canine n 8-min freezing group,and cystic changes in the femur heads were further observed in some model canines;while the slight osteosclerosis in femur heads was observed in only 2 model canines in4-min freezing

关 键 词：股骨头坏死 模型 动物 犬 液氮 

分 类 号：R681.8[医药卫生—骨科学]