PDGFRαreporter activity identifies periosteal progenitor cells critical for bone formation and fracture repair  

作　　者：Jiajia Xu Yiyun Wang Zhu Li Ye Tian Zhao Li Amy Lu Ching-Yun Hsu Stefano Negri Cammy Tang Robert J.Tower Carol Morris Aaron W.James 

机构地区：[1]Departments of Pathology,Johns Hopkins University,Baltimore,MD 21205,USA [2]Departments of Orthopedics,Johns Hopkins University,Baltimore,MD 21205,USA [3]Department of Oral and Maxillofacial Surgery,School of Stomatology,China Medical University,Shenyang,Liaoning,PR China

出　　处：《Bone Research》2022年第1期122-136,共15页骨研究（英文版）

基　　金：supported by the NIH/NIAMS (R01 AR070773);NIH/NIDCR (R21 DE027922);the Department of Defense (W81XWH-18-1-0121, W81XWH-18-1-0336, W81XWH-1810613, W81XWH-20-1-0302);the American Cancer Society (Research Scholar Grant, RSG-18-027-01-CSM);the Maryland Stem Cell Research Foundation;MTF Biologics

摘　　要：The outer coverings of the skeleton,which is also known as the periosteum,are arranged in concentric layers and act as a reservoir for tissue-specific bone progenitors.The cellular heterogeneity within this tissue depot is being increasingly recognized.Here,inducible PDGFRαreporter animals were found to mark a population of cells within the periosteum that act as a stem cell reservoir for periosteal appositional bone formation and fracture repair.During these processes,PDGFRαreporter^(+)progenitors give rise to Nestin+periosteal cells before becoming osteoblasts and osteocytes.The diphtheria toxin-mediated ablation of PDGFRαreporter^(+)cells led to deficits in cortical bone formation during homeostasis and a diminutive hard callus during fracture repair.After ossicle transplantation,both mouse PDGFRαreporter^(+)periosteal cells and human Pdgfrα+periosteal progenitors expand,ossify,and recruit marrow to a greater extent than their counterpart periosteal cells,whereas PDGFRαreporter^(−)periosteal cells exhibit a predisposition to chondrogenesis in vitro.Total RNA sequencing identified enrichment of the secreted factors Fermt3 and Ptpn6 within PDGFRαreporter^(+)periosteal cells,which partly underlie the osteoblastogenic features of this cell population.

关 键 词：PROGENITOR FRACTURE RESERVOIR 

分 类 号：R68[医药卫生—骨科学]