外泌体功能化串晶结构纤维膜的制备及其成骨分化性能  

Preparation of exosome-functionalized shish-kebab fibrous membrane and its osteogenic differentiation ability

作　　者：张宇 刘来俊 李超婧 晋巧巧 谢千阳 李佩伦[3,6] 王富军 王璐[1,2] ZHANG Yu;LIU Laijun;LI Chaojing;JIN Qiaoqiao;XIE Qianyang;LI Peilun;WANG Fujun;WANG Lu(Key Laboratory of Textile Science & Technology, Ministry of Education, Donghua University, Shanghai 201620, China;College of Textiles, Donghua University, Shanghai 201620, China;College of Stomatology, Shanghai Jiaotong University, Shanghai 200011, China;Department of Endodontics, Ninth People′s Hospital, Shanghai Jiaotong University School of Medicine, Shanghai 200011, China;Department of Oral Surgery, Ninth People′s Hospital, Shanghai Jiaotong University School of Medicine, Shanghai 200011, China;Department of Orthodontics, Ninth People′s Hospital, Shanghai Jiaotong University School of Medicine, Shanghai 200011, China)

机构地区：[1]东华大学纺织面料技术教育部重点实验室,上海201620 [2]东华大学纺织学院,上海201620 [3]上海交通大学口腔医学院,上海200011 [4]上海交通大学医学院附属第九人民医院牙体牙髓科,上海200011 [5]上海交通大学医学院附属第九人民医院口腔外科,上海200011 [6]上海交通大学医学院附属第九人民医院口腔正畸科,上海200011

出　　处：《纺织学报》2022年第3期24-30,37,共8页Journal of Textile Research

基　　金：高等学校学科创新引智计划2.0项目(BP0719035);东华大学研究生创新基金资助项目(GSIF-DH-M-2021001)。

摘　　要：为提高合成可吸收引导骨再生膜的骨诱导活性,通过静电纺丝和溶剂诱导结晶法制备了聚己内酯(PCL)/β-磷酸三钙(β-TCP)串晶纳米纤维膜,并通过聚多巴胺的黏附作用将外泌体负载于纤维膜上进行改性,对复合纤维膜的微观形貌、化学组成、理化性能和细胞成骨分化性能进行测试与分析。结果表明:PCL/β-TCP纳米纤维上成功诱导出串晶结构,且经串晶和聚多巴胺双重修饰的纤维膜具有最佳的表面浸润性和优异的蛋白吸附能力,最终获得的外泌体功能化的串晶纳米纤维膜在串晶结构、聚多巴胺和外泌体共同作用下可促进骨髓间充质干细胞碱性磷酸酶活性的增加,有望应用于体内加速骨愈合。In order to improve the osteoconduction of synthetic resorbable membranes for guided bone regeneration,polycaprolactone(PCL)/β-tricalcium phosphate(β-TCP)shish-kebab nanofibrous membranes were prepared by electrospinning and solvent-induced crystallization.Exosomes were then immobilized on membranes via adhesion of polydopamine.The morphology,chemical composition,physicochemical and cellular osteogenic differentiation properties of the composite fibrous membranes were characterized.The shish-kebab structure was successfully induced on PCL/β-TCP nanofibers.The results show that membranes doubly modified by shish-kebab and polydopamine had the best surface wettability and excellent protein adsorption ability.Exosome-functionalized shish-kebab fibrous membrane increases the alkaline phosphatase activity of bone marrow mesenchymal stem cells due to the synergistic effect of shish-kebab structure,polydopamine,and exosome,which has good therapeutic potential for promoting bone healing in vivo.

关 键 词：纳米纤维膜 引导骨再生 静电纺丝 串晶纤维膜 外泌体 多巴胺 聚己内酯 骨组织修复材料 

分 类 号：TS101.4[轻工技术与工程—纺织工程]