饲粮蛋白质水平对妊娠期云南半细毛羊氮平衡和养分排放的影响  

Effects of Dietary Crude Protein Level on Nitrogen Balance and Nutrient Excretions of Yunnan Semi-Fine Wool Ewes during Pregnancy

作　　者：赵小琪 王思宇 薛白[2] 倪晓君 李银江 洪琼花 ZHAO Xiaoqi;WANG Siyu;XUE Bai;NI Xiaojun;LI Yinjiang;HONG Qionghua(Yunnan Animal Science and Veterinary Institute,Kunming 650224,China;Institute of Animal Nutrition,Sichuan Agricultural University,Chengdu 611130,China)

机构地区：[1]云南省畜牧兽医科学院,昆明650224 [2]四川农业大学动物营养研究所,成都611130

出　　处：《动物营养学报》2022年第3期1709-1720,共12页CHINESE JOURNAL OF ANIMAL NUTRITION

基　　金：国家绒毛用羊产业技术体系(CARS39-08);云南省科技创新人才计划(2018HC017);云南省科技人才和平台计划(2018IC108)。

摘　　要：本试验旨在研究饲粮蛋白质水平对妊娠期云南半细毛羊氮平衡和氮、磷、钙排放的影响。选取50只体况良好、体重[(46.52±4.58)kg]相近、年龄一致、配种日期相同的经产2胎妊娠期云南半细毛羊,随机分为5组,每组10个重复,每个重复1只羊。5组试验羊在妊娠前期和中期饲喂蛋白质水平分别为6.65%(Ⅰ组)、9.02%(Ⅱ组)、10.97%(Ⅲ组)、12.43%(Ⅳ组)和13.95%(Ⅴ组)的试验饲粮,妊娠后期饲喂蛋白质水平分别为6.31%(Ⅰ组)、7.77%(Ⅱ组)、10.47%(Ⅲ组)、12.69%(Ⅳ组)和15.25%(Ⅴ组)的试验饲粮。从妊娠第46天算起,所有试验羊进行为期104 d的饲养试验,其中预试期14 d,正试期90 d。分别在妊娠前期(妊娠第71~75天)、中期(妊娠第101~105天)和后期(妊娠第131~135天)从各组选5只试验羊进行为期5 d的消化代谢试验。结果表明:1)在妊娠前期,饲粮蛋白质水平对粪干物质排放量、尿磷排放量、尿钙排放量的影响不显著(P>0.05);粪氮排放量、尿氮排放量和氮总排放量随着饲粮蛋白质水平的升高而增加;Ⅴ组的粪磷排放量、磷总排放量显著高于Ⅰ组(P<0.01);Ⅴ组的粪钙排放量、钙总排放量最高,而Ⅲ组最低。在妊娠中期,粪干物质排放量、尿磷排放量不受饲粮蛋白质水平的显著影响(P>0.05);尿氮排放量、氮总排放量、粪磷排放量、磷总排放量随着饲粮蛋白质水平的升高而增加,Ⅳ的粪氮排放量、粪钙排放量、钙总排放量最高,Ⅰ组的尿钙排放量显著高于其他各组(P<0.05)。在妊娠后期,饲粮蛋白质水平对粪干物质排放量、尿磷排放量的影响不显著(P>0.05);粪氮排放量、尿氮排放量、氮总排放量、粪磷排放量、磷总排放量、粪钙排放量随着饲粮蛋白质水平的升高而增加。2)妊娠前期和中期云南半细毛羊氮排放以粪中排放为主,而在妊娠后期,当采食氮较低时,氮排放仍然以粪中排放为主,但随着采食氮的增加,尿氮排放�This experiment aimed to study the effects of dietary protein level on nitrogen balance and nitrogen,phosphorus and calcium excretions of Yunnan semi-fine wool ewes during pregnancy.Fifty multiparous Yunnan semi-fine wool ewes with good body condition,similar body weight[(46.52±4.58)kg],the same age,and the same mating date were randomly divided into 5 groups with 10 replicates per group and 1 ewe per replicate.The experimental diets with the protein levels of 6.65%(groupⅠ),9.02%(groupⅡ),10.97%(groupⅢ),12.43%(groupⅣ)and 13.95%(groupⅤ)were fed in pre-and mid-pregnancy,and the experimental diets with the protein levels of 6.31%(groupⅠ),7.77%(groupⅡ),10.47%(groupⅢ),12.69%(groupⅣ)and 15.25%(groupⅤ)were fed in late-pregnancy.From the 46th day of pregnancy and lasted for 104 days,including 14 days preliminary period and 90 days formal period.Five sheep were selected from each group for a 5-day digestion and metabolism trail in pre-pregnancy(the 71st to 75th day of pregnancy),mid-pregnancy(the 101st to 105th day of pregnancy)and late-pregnancy(the 131st to 135th day of pregnancy).The results showed as follows:1)in pre-pregnancy,dietary protein level had no significant effects on fecal dry matter excretion,urinary phosphorus excretion and urinary calcium excretion(P>0.05);the fecal nitrogen excretion,urinary nitrogen excretion and total nitrogen excretion were increased with the increase of dietary protein level;the fecal phosphorus excretion and total phosphorus excretion of groupⅤwere significantly higher than groupⅠ(P<0.01);the fecal calcium excretion and total calcium excretion of groupⅤwere the highest and groupⅢwas the lowest.In mid-pregnancy,fecal dry matter excretion and urinary phosphorus excretion were not significantly affected by dietary protein level(P>0.05);the urinary nitrogen excretion,total nitrogen excretion,fecal phosphorus excretion and total phosphorus excretion were increased with the increase of dietary protein level.Fecal nitrogen excretion,fecal calcium excretion and

关 键 词：云南半细毛羊 妊娠期 蛋白质 氮平衡 养分排放 

分 类 号：S816[农业科学—饲料科学]