种植模式和施肥处理下根际土壤碳源利用能力的研究  

Effects of planting patterns and fertilization treatments on carbon source utilization capacity of rhizosphere soil

作　　者：于海玲[1] 张晓岩 李晓宇 蔡万美 高强[1] YU Hai-ling;ZHANG Xiao-yan;LI Xiao-yu;CAI Wan-mei;GAO Qiang(School of Resources and Environment,Jilin Agricultural University,Changchun 130118,China)

机构地区：[1]吉林农业大学资源与环境学院,吉林长春130118

出　　处：《东北师大学报（自然科学版）》2022年第1期126-133,共8页Journal of Northeast Normal University(Natural Science Edition)

基　　金：吉林省自然科学基金资助项目(20180101126JC);吉林省教育厅“十三五”产业化项目(JJKH20200334KJ).

摘　　要：为阐明不同种植模式和施肥处理对根际土壤微生物活性的影响,采集单作玉米和花生、间作玉米和花生根际土壤,利用群落水平生理指纹图谱培养技术,探究不同处理下根际微生物碳源代谢活性的差异.分析结果表明,种植模式改变引起土壤中微生物组成丰富度的变化.间作提高了花生土壤的平均颜色随时间变化率(AWCD)值,降低了玉米土壤的AWCD值.进一步间作提高了花生的碳水化合物、氨基酸类、羧酸和多聚物的利用能力,玉米反之.双因素方差分析表明种植模式和施肥处理显著影响了玉米、花生中微生物对糖类和羧酸类物质代谢能力,而酚酸类物质仅受施肥处理的影响.主成分分析表明,单作和间作模式对玉米和花生土壤微生物的碳源利用能力有显著差异.总之,种植模式和施肥处理及相互作用对土壤碳源利用能力有显著影响,间作种植体系增强了土壤微生物群落多样性,进而改变了根际土壤微生物碳源利用能力和种类.In order to elucidate the effects of different planting patterns and fertilization treatments on microbial activity in rhizosphere soil, the rhizosphere soils of monocropped maize and peanut, intercropped maize and flower were collected, and community level physiological finger printing(Biology) culture technique was used to investigate the differences in carbon source metabolic activity of rhizosphere microorganisms under different treatments.The results showed that the change of planting pattern caused the change of microbial composition richness in soil.Intercropping increased the average color change rate over time(AWCD) of peanut soil and decreased the AWCD of corn soil.The utilization capacity of carbohydrate, amino acid, carboxylic acid and poly of peanut was further improved by intercropping, while the utilization capacity of carbohydrate, amino acid, carboxylic acid and poly was decreased by intercropping of maize.Principal component analysis showed that there was a significant difference between monocropping and intercropping patterns in the utilization of soil microbial carbon sources.In conclusion, the intercropping system enhanced the soil microbial community diversity, and then changed the rhizosphere soil microbial carbon source utilization capacity and species.

关 键 词：间作 施肥 根际微生物 碳源利用率 

分 类 号：S154.3[农业科学—土壤学]