Arabidopsis NLP7 improves nitrogen use efficiency and yield in cotton  

作　　者：JAN Sami Ullah LIAQAT Ayesha ZHU Yonghong LI Jing ZHANG Huangyang ABDALLA Mohnad WU Jie XIANG Chengbin WU Shenjie ALFATIH Alamin 

机构地区：[1]School of Life Sciences,Division of Molecular&Cell Biophysics,Hefei National Science Center for Physical Sciences at the Microscale,University of Science and Technology of China,The Innovative Academy of Seed Design,Chinese Academy of Sciences,Hefei,Anhui 230026,China [2]Institute of Cotton Research,Shanxi Agricultural University,Yuncheng 044000,China [3]College of Life Sciences,Shanxi Agricultural University,Taiyuan 030031,China

出　　处：《Journal of Cotton Research》2022年第1期12-21,共10页棉花研究（英文）

基　　金：supported by grants from Ministry of Science and Technology of China(Grant No.2016ZX08005004-003).

摘　　要：Background: Nitrogen(N) is a required macronutrient for cotton growth and productivity. Excessive N fertilizers are applied in agriculture for crop yield maximization, which also generates environmental pollution. Improving crop N use efficiency(NUE) is the most economical and desirable way of reducing fertilizer application and environmental pollution. NUE has been an important issue in cotton. So far there is no report on cotton NUE improvement via transgenic approach. Nin-like proteins(NLP) are transcription factors regulating NUE. We previously demonstrated that At NLP7 improved NUE and biomass when overexpressed in Arabidopsis. However, it is not known whether At NLP7 can be used to improve NUE in crops.Results: To test the feasibility, we expressed At NLP7 in cotton and evaluated NUE and yield of the transgenic cotton in the field. Transgenic cotton showed improved NUE and yield under both low and high N conditions. In addition, plant biomass, amount of absorbed N, N contents, activities of N-assimilating enzymes, and the expression of N-related marker genes were significantly increased in transgenic cotton compared with the wild type control, suggesting that At NLP7 enhances NUE in cotton.Conclusion: Together, our results demonstrate that At NLP7 is a promising candidate to improve NUE and yield in cotton.

关 键 词：Nitrogen Nitrogen metabolism Nitrogen use efficiency(NUE) Nin-like protein(NLP) AtNLP7 Cotton(Gossypium hirsutum) 

分 类 号：S562[农业科学—作物学]