电针对骨癌痛大鼠脊髓星形胶质细胞活化及炎性因子的影响  

Effects of Electroacupuncture on the Activation of Spinal Astrocytes and Inflammatory Factors in Rats with Cancer-induced Bone Pain

作　　者：王凤娇 具紫勇[1] 范神栋 陈灏[2] 施舍 王功命 梁嘉仪 王珂 夏勇[1] WANG Fengjiao;JU Ziyong;FAN Shendong;CHEN Hao;SHI She;WANG Gongming;LIANG Jiayi;WANG Ke;XIA Yong(Shanghai University of Traditional Chinese Medicine,Shanghai 201203,China;Fudan University Shanghai Cancer Center,Shanghai 200032,China;Yueyang Hospital of Integrated Traditional Chinese and Western Medicine,Shanghai University of Traditional Chinese Medicine,Shanghai 200437,China)

机构地区：[1]上海中医药大学,上海201203 [2]复旦大学附属肿瘤医院,上海200032 [3]上海中医药大学附属岳阳中西医结合医院,上海200437

出　　处：《上海针灸杂志》2022年第3期291-296,共6页Shanghai Journal of Acupuncture and Moxibustion

基　　金：国家自然科学基金面上项目(81874506);上海市教育委员会预算内项目(2019LK079);国家自然科学基金项目(81673756);上海市卫生计生系统优秀学科带头人(2018BR24);上海市科学技术委员会医学引导项目(17401930700)。

摘　　要：目的探讨电针对骨癌痛(CIBP)大鼠的镇痛效应及对脊髓星形胶质细胞活化和炎性因子表达的影响。方法SD雌性大鼠随机分为空白组、假手术组、模型组和电针组,每组12只。模型组和电针组于胫骨髓腔内注射10μL Walker256乳腺癌细胞,假手术组则注射10μL磷酸盐缓冲液(PBS)。电针组于造模后第11天开始进行电针干预,隔日1次,共3次。测定大鼠机械痛阈变化。HE染色检测大鼠胫骨组织形态学变化,Western blot法测定L3~L5脊髓背角星形胶质细胞标记物胶质原纤维酸性蛋白(GFAP)的表达,ELISA法测定L3~L5脊髓背角肿瘤坏死因子(TNF)-α、白细胞介素(IL)-1β、IL-6的含量。结果与空白组和假手术组比较,模型组大鼠的机械痛阈显著降低(P<0.05);与模型组比较,电针组机械痛阈显著升高(P<0.05)。HE染色结果显示模型组新生骨组织纹理模糊不清,软骨细胞柱遭到破坏,吸收凹陷内可见大量癌细胞;电针组过渡性骨小梁之间可见大量癌细胞浸润;空白组和假手术组均未见异常。与空白组和假手术组相比,模型组大鼠脊髓背角星形胶质细胞标记蛋白GFAP的表达增加(P<0.05);与模型组相比,电针干预可显著逆转其增加(P<0.05)。与空白组和假手术组比较,模型组L3~L5脊髓背角IL-1β、IL-6的含量升高(P<0.05);与模型组比较,电针干预可降低IL-1β、IL-6的表达(P<0.05)。各组大鼠脊髓背角TNF-α的表达比较差异无统计学意义(P>0.05)。结论电针对骨癌痛大鼠具有镇痛效应,其可能通过抑制脊髓背角星形胶质细胞活化诱导的炎症反应来发挥作用。Objective To discover the analgesic effect of electroacupuncture(EA)on rats with cancer-induced bone pain(CIBP)and its effects on the activation of spinal astrocytes and the expression of inflammatory factors.Method Female Sprague-Dawley rats were randomized into a blank control group,a sham operation group,a model group,and an EA group,with 12 rats in each group.The model and EA groups received injection of 10μL Walker256 breast cancer cells into the tibial medullary cavity,and the sham operation group received injection of 10μL phosphate buffer solution(PBS).The EA intervention started from the 11th day after the modeling in the EA group,once every other day for three times.The mechanical pain threshold was measured for the rats.Hematoxylin and eosin(HE)staining was used to observe the histomorphological changes of the tibial bone tissue.Western blot(WB)was adopted to determine the expression of the spinal astrocyte marker glial fibrillary acidic protein(GFAP).ELISA was employed to detect the contents of tumor necrosis factor(TNF)-α,interleukin(IL)-1β,and IL-6 in the L3-L5 spinal cord dorsal horn.Result Compared with the blank control and sham operation groups,the mechanical pain threshold declined significantly in the model group(P<0.05).The mechanical pain threshold increased markedly in the EA group compared with the model group(P<0.05).The HE staining revealed blurry new bone tissue and damaged chondrocytes in the model group,and a large number of cancer cells in the depression dips.A large amount of cell cancer infiltration was discovered in the transitional trabecular bones in the EA group.Abnormal findings were not found in the blank control group or sham operation group.Compared with the blank control and sham operation groups,the expression level of spinal astrocyte marker GFAP increased in the model group(P<0.05),and the EA intervention significantly reverted the increase compared with the model group(P<0.05).Compared with the blank control and sham operation groups,the contents of IL-1βand IL-6

关 键 词：电针 癌症疼痛 肿瘤坏死因子-α 白细胞介素-1Β 白细胞介素-6 星形胶质细胞 大鼠 

分 类 号：R2-03[医药卫生—中医学]