甘肃野生羊肚菌根际土壤真菌群落与环境因子相互关系  

Relationship between Fungal Community and Environmental Factors in Rhizosphere Soil around Wild Morels in Gansu

作　　者：赵玉卉[1] 郭瑞[1] 杨阿丽 秦鹏[1] 金辉[2] 魏甲乾[1] 冉永红 陈志荣 路等学[1] ZHAO Yu-hui;GUO Rui;YANG A-li;QIN Peng;JIN Hui;WEI Jia-qian;RAN Yong-hong;CHEN Zhi-rong;LU Deng-xue(Inst.of Biol.,Gansu Acad.of Sci.,Lanzhou 730000;CAS Lanzhou Inst.of Chemo-Physics,Key Lab.of NW Characteristics Plant Res.Chem.,Lanzhou 730000;Dingxi City Acad.of Agric.Sci.,Dingxi 743000;Coll.of Bio-Med.Wenzhou Uni.of Med.,Wenzhou 325000)

机构地区：[1]甘肃省科学院生物研究所,甘肃兰州730000 [2]中国科学院兰州化学物理研究所中国科学院西北特色植物资源化学重点实验室,甘肃兰州730000 [3]定西市农业科学研究院,甘肃定西743000 [4]温州医科大学生物医学工程学院,浙江温州325000

出　　处：《微生物学杂志》2022年第1期96-106,共11页Journal of Microbiology

基　　金：国家自然科学基金项目(31560037);甘肃省自然科学基金项目(21JR1RA348);甘肃省科学院应用研究与开发项目(2017JK-05,2018JK-06);甘肃省科学院农牧废弃物生物资源化创新团队项目(2019CX004-01);甘肃省委组织部陇原青年创新创业人才项目(2020RCXM146)。

摘　　要：为探究甘肃不同采样点野生羊肚菌根际土壤中真菌群落结构以及真菌群落与环境因子的相关性,采用Illumina Mi-Seq高通量测序技术和生物信息学方法,分析真菌菌群组成和多样性,揭示真菌群落与环境因子的相互关系。结果表明,不同采样点的根际土壤中真菌群落差异较大;9个采样点共有的OTU(operational taxonomic unit)数为32个,特有的OTU数较多;迭部采样点羊肚菌根际土壤真菌多样性最高。所有采样点的优势菌门均为子囊菌门(Ascomycete)和担子菌门(Basidiomycota,),而纲、科、属水平优势类群各异。冗余分析(redundancy analysis,RDA)表明,子囊菌门与速效钾、谷氨酰胺酶、脲酶、硝态氮、碱性磷酸酶和脱氢酶箭头方向一致,说明子囊菌门与速效钾、谷氨酰胺酶、脲酶、硝态氮、碱性磷酸酶和脱氢酶正相关;Rozellomycota和Olpidiomycota与硒、铁、有效磷、氨态氮、脱氢酶呈正相关,与钙、多酚氧化酶、镁、pH呈负相关;壶菌门、unclassified_Fungi、Mortierellomycota与脱氢酶、碱性磷酸酶、硝态氮呈负相关。野生羊肚菌根际土壤真菌群落受酶活性影响较理化性质的影响大。This study aiming at exploring the reciprocity between fungal community structure as well as fungal community and environmental factors(FC-SEF)of wild morels in different sampling spots in Gansu Province.Illumina Mi-Seq high-throughput sequencing technology and informatics method were adopted to analyze the composition and diversity of fungal community,and reveal the reciprocity between fungal community and environmental factors.The results showed that there was wide difference among rhizosphere fungi from different sampling spots;there were a total of 32 OTU(operational taxonomic unit)from nine sampling spots,there were more peculiar OTU there;the diversity of morel rhizosphere soil fungi in overlapping sampling points was the highest.The dominant fungal phylum of all sampling spots was subphylum of ascomycetes and subphylum of basidiomycetes,and the dominant groups at class,family,and species level differ from each other.Redundancy analysis showed that the subphylum of ascomycetes accorded with the direction of arrow point of quick-acting potassium,glutaminase,urease,nitrate nitrogen,alkaline phosphatase,and dehydrogenase,showed that the subphylum of ascomycetes was positive correlation with quick-acting potassium,glutaminase,urease,nitrate nitrogen,alkaline phosphatase,and dehydrogenase.Rozellomycota and Olpidiomycota are positively correlated with Se,Fe content,available phosphorus,ammonia nitrogen,and dehydrogenase,but are negatively correlated with Ca,Mg content,pH value,and Peroxidase.Chytridiomycota,unclassified Fungi,and Mortierellomycota are negatively correlated with dehydrogenase,alkaline phosphatase and nitrate.Fungal communities in the rhizosphere soil of wild morels are more affected by enzyme activity than by physical and chemical factors.

关 键 词：野生羊肚菌 酶活性 理化性质 真菌群落 高通量测序 

分 类 号：Q938.11[生物学—微生物学]