HPLC法测定香莲祛痛霜中血竭素含量  

Determination of Dracodrin in Xiang Lian Qu Tong Cream by High-Performance Liquid Chromatography

作　　者：陈依春 孙芳云 张芳侠 郝婷婷 李捷 朱爱华 CHEN Yichun;SUN Fangyun;ZHANG Fangxia;HAO Tingting;LI Jie;ZHU Aihua(Engineering Research Center of Tibetan Medicine Detection Technology,Ministry of Education,Basic Research Laboratory of Life Science and Tibetan Medicine Screening Laboratory of Basic Medical College of Tibetan University for Nationalities,Xianyang 712082,China;Shanxi Institute of Traditional Chinese Medicine,Xi'an 712000,China)

机构地区：[1]藏药检测技术教育部工程研究中心、西藏民族大学基础医学院生命科学基础研究实验室、藏药筛选实验室,陕西咸阳712082 [2]陕西中药研究所,陕西西安712000

出　　处：《中国民族民间医药》2022年第5期42-45,共4页Chinese Journal of Ethnomedicine and Ethnopharmacy

基　　金：西藏自治区科技厅-香莲祛痛霜Ⅱ期临床试验研究(No.2015xzo1G62);陕西省科技统筹创新工程计划(2015KTCQ03-07)。

摘　　要：目的:采用高效液相色谱(HPLC)法建立香莲祛痛霜血竭素含量的测定方法。方法:按照HPLC法(中国药典2015年版四部通则)测定。色谱条件与系统适用性试验色谱条件。色谱柱:AgilentC 18(250 mm×4.6 mm,5μm);流动相:乙腈-0.05 mol/L磷酸二氢钠溶液(45∶55);检测波长:440 nm;流速:1 mL/min;柱温:40℃;进样体积:10μL。结果:血竭素对照品溶液,以浓度对峰面积进行回归,在5.817~58.17μg/mL之间呈线形关系,平均峰面积675.2,RSD为0.35%;香莲祛痛霜平均峰面积314.7,RSD为1.36%;稳定性试验血竭素高氯酸盐对照品溶液均峰面积674.6,RSD为0.25%,样品中血竭素高氯酸盐平均峰面积362.2,RSD为2.32%;血竭素高氯酸盐的平均回收率为97.8%,RSD(n=9)为2.29%;三组不同批次的样品1 g血竭素含量为0.03248%。结论:本方法简便易行,结果准确,重复性好,可作为香莲祛痛霜质量控制标准使用。Objective To establish a High-Performance Liquid Chromatography(HPLC)method for the determination of dracodrin in Xiang Lian Qu Tong cream.Methods According to the four general principles of Chinese Pharmacopoeia 2015 edition.Chromatographic Conditions and System Applicability Test Chromatographic Conditions.Chromatographic Column:Agilent C 18(250 mm×4.6 mm,5μm);Mobile Phase:Acetonitrile-0.05 mol/L Sodium Dihydrogen Phosphate Solution(45∶55);Detection Wavelength:440 nm;Flow Rate:1 mL/min;Column;Temperature:40℃;Injection:10μL.Results dracorhodin reference solution showed a linear relationship between 5.817 and 58.17μg/mL,with an average peak area of 675.2 and RSD of 0.35%.The average peak area of Xianglian Qutong Cream was 314.7 and RSD was 1.36%.The average peak area of dracorhodin perchlorate reference solution was 674.6 and RSD was 0.25%.The average peak area of dracorhodin perchlorate was 362.2,RSD was 2.32%.The average recovery rate of dracorhodin perchlorate was 97.8%,RSD(n=9)was 2.29%.The content of dracorhodin in 1 g of three groups of samples was 0.03248%.Conclusion The method is simple,accurate and reproducible.It can be used as the quality control standard of Xianglian Qutong Cream.

关 键 词：香莲祛痛霜 血竭素 高效液相色谱 质量标准 

分 类 号：R284.1[医药卫生—中药学]