Determination of amphetamines,ketamine and their metabolites in hair with high-speed grinding and solid-phase microextraction followed by LC-MS  

作　　者：Liang Meng Yong Dai Chen Chen Jun Zhang 

机构地区：[1]Department of Forensic Science,Fujian Police Collge,Fuzhou,China [2]Engineering Research Center,Fujian Police College,Fuzhou,China [3]Department of Forensic Science,Sichuan Police College,Luzhou,China [4]Drug Research Center of Integrated Traditional Chinese and Western Medicine,Affliated Traditional Chinese Medicine Hospital of Southwest Medical University,Luzhou,China

出　　处：《Forensic Sciences Research》2021年第4期273-280,共8页法庭科学研究（英文）

基　　金：Financial supports from Major Project of Sichuan Provincial Public Security Department[grant number 201901];Support Program of Outstanding Talents in the New Century in Universities of Fujian,China,Young Scientific Natural Science Foundation of Universities in Fujian,China and Funding for High-Level Talent of Luzhou Municipal People's Government-Southwest Medical University are gratefully acknowledged.

摘　　要：A novel hair sample pre-treatment method based on high-speed grinding and solid-phase microextraction(SPME)had been applied for the determination of amphetamines,ketamine and their metabolites in hair samples by liquid chromatography mass spectrometry(LC-MS).A 20mg sample of hair was ground with 2 mL of saturated sodium carbonate solution using a high-efficiency hair grinder with 70 Hz osillation for 2min at 4℃.After centrifuging,1.5mL of the supernatant was transferred and treated with SPME by direct immersion(DI-SPME).The target analytes extracted by fibre were desorbed and analysed using LC-MS.Under the optimum conditions,a recovery of 90.2%-95.8%was obtained for all analytes.The analytical method was linear for all analytes in the range from 0.2 to 10 ng/mg with the correlation coefficient ranging from 0.9985 to 0.9993.The detection limits for all analytes were estimated to be 0.067 ng/mg.The accuracy(mean relative error)was within±6.9%and the precision(relative standard error)was less than 6.8%.The combination of high-speed grinding of hair and SPME had the advantages of being easy to perform,environment-friendly and high in detection sensitivity.The proposed method offered an altermative ana lytical approach for the sensitive detection of drugs in hair samples for forensic purposes.

关 键 词：Forensic sciences forensic toxicology drugs of abuse high-speed grinding hair analysis liquid chromatography-mass spectrometry solid-phase microextraction 

分 类 号：D919.2[医药卫生—法医学]