基于转录组测序挖掘小麦叶片抗旱相关基因  

Screening of Candidate Genes Resistant to Drought Stress in Wheat Leaves Based on Transcriptome Sequencing

作　　者：曾倩倩 刘岩 朱墨 邱宗波[1] ZENG Qianqian;LIU Yan;ZHU Mo;QIU Zongbo(College of Life Sciences,Henan Normal University/Henan International Joint Laboratory of Agricultural Microbial Ecology and Technology,Xinxiang 453007,China)

机构地区：[1]河南师范大学生命科学学院/河南省农业微生物生态与技术国际联合实验室,河南新乡453007

出　　处：《广东农业科学》2022年第2期1-8,共8页Guangdong Agricultural Sciences

基　　金：国家自然科学基金(31500499);河南省高校科技创新人才项目(16HASTIT019)。

摘　　要：【目的】通过转录组测序分析干旱胁迫的小麦,以期挖掘小麦响应干旱胁迫的关键基因。【方法】采用高通量Illumina Hiseq 2500测序平台对郑麦366号正常生长与自然干旱处理进行测序,并对测序数据进行分析,筛选响应干旱胁迫的关键基因。【结果】两组样品中分别获得45471018、45133470个clean read片段,包含6.82 Gb、6.39 Gb碱基信息,拼接组装后,得到188334个转录本和119588个Unigenes。通过筛选共获得1207个DEGs,其中752个上调,455个下调。GO功能富集分析显示,上调的DEGs主要富集在水解酶活性、催化活性、代谢过程、甘油代谢过程、膜和细胞部分等;下调的DEGs富集在细胞器、细胞和氧化还原酶活性的最多。KEGG途径富集分析发现,DEGs显著富集于淀粉和蔗糖代谢、卟啉和叶绿素代谢、光合作用-天线蛋白、光合作用、苯丙烷生物合成、乙醛酸和二羧酸代谢、甘油磷脂代谢、氨基酸代谢和光合生物中的碳固定等代谢通路。5个抗氧化酶基因的qRT-PCR结果与RNA-Seq测序结果变化趋势相一致。【结论】利用转录组测序技术获得了大量小麦抗旱相关基因,为深入研究小麦响应干旱胁迫的分子机制提供更多的基因遗传资源。【Objective】The study was carried out to identify key genes in response to drought stress in wheat through transcriptome sequencing.【Method】By using Illumina HiSeq 2500 sequencing platform,sequencing was employed to Zhengmai 366 exposed to the control and natural drought stress,and the sequencing data were analyzed.【Result】45471018 and 45133470 clean reads were obtained from the two sets of samples,containing 6.82 Gb and 6.39 Gb base information,respectively.After splicing and assembly,188334 transcripts and 119588 Unigenes were obtained.A total of 1207 DEGs were screened,of which 752 were up-regulated and 455 were down-regulated.GO functional enrichment analysis showed that the up-regulated DEGs were mainly enriched in hydrolase activity,catalytic activity,metabolic process,glycerol metabolism,membrane and cell parts,etc.;however,down-regulated DEGs were mostly enriched in organelles,cells and oxidoreductase activities.KEGG pathway enrichment analysis showed that DEGs were significantly enriched in starch and sucrose metabolism,porphyrin and chlorophyll metabolism,photosynthesis-antenna protein,photosynthesis,phenylpropane biosynthesis,glyoxylic acid and dicarboxylic acid metabolism,glycerophospholipid metabolism,amino acid metabolism and carbon f ixation in photosynthetic organisms.The qRT-PCR results of 5 antioxidant enzyme genes were consistent with RNA-Seq sequencing results.【Conclusion】A large number of wheat drought-resistant genes are obtained by transcriptome sequencing technology,which provides more genetic resources for further study of the molecular mechanism of wheat response to drought stress.

关 键 词：小麦 干旱胁迫 RNA-Seq技术 GO功能富集 KEGG代谢通路 

分 类 号：S512.1[农业科学—作物学]