固定化脱氮菌剂处理垃圾渗滤液尾水研究  

Study on Treatment of Landfill Leachate Tail Water with Immobilized Denitrifying Bacteria

作　　者：武夏溦 湛小静 余佳芯 陈聪[1] WU Xiawei;ZHAN Xiaojing;YU Jiaxin;CHEN Cong(College of Ecology and Environment,Chengdu University of Technology,Chengdu 610059,China)

机构地区：[1]成都理工大学生态环境学院,四川成都610059

出　　处：《生物化工》2022年第1期12-17,共6页Biological Chemical Engineering

摘　　要：对2株从垃圾渗滤液中提取到的好氧反硝化菌株PM02和PR07进行形态学鉴定和16S rDNA序列分析,2个菌株分别为摩氏假单胞菌和树脂假单胞菌。对以海藻酸钠(SA)为包埋材料、复合菌比例为PM02∶PR07=1∶1的固定化小球进行垃圾渗滤液尾水处理的初步研究。结果表明:最佳包埋固定化条件为3%SA、4%CaCl_(2)、交联时间18 h,在pH=7.0、投加量为22.99 g/L、温度25℃条件下固定化小球对NO_(3)^(-)-N、TN的去除效率分别达到了98.48%、76.91%;在此条件下,处理模拟渗滤液尾水,对NO_(3)^(-)-N的去除率能达到100%,比游离菌剂高出了5.82个百分点。固定化复合菌剂性能出众、脱氮效率高。Morphological identification and 16S rDNA sequence analysis of two aerobic denitrifying strains PM02 and PR07 extracted from landfill leachate.And use sodium alginate as the embedding material,the composite bacteria ratio is PM02:PR07=1:1 immobilized pellets for preliminary research.The results showed that the two strains were Pseudomonas mosselii and Pseudomonas resinovorans,and the best embedding and immobilization process conditions were 3%SA,4%CaCl_(2),18 h cross-linking time,and at pH=7.0,dosage of 22.99 g/L,and temperature of 25℃,the removal efficiency of NO_(3)^(-)-N and TN by immobilized beads reached 98.48%and 76.91%.Under these conditions,the removal rate of NO_(3)^(-)-N can reach 100%by treating the tail water of the simulated leachate,which is 5.82%higher than that of the free bacterial agent.The immobilized composite bacterial agent has outstanding performance and high nitrogen removal efficiency.

关 键 词：好氧反硝化菌 复合菌 固定化 垃圾渗滤液尾水 

分 类 号：X703[环境科学与工程—环境工程]