γ射线照射对肺泡上皮细胞生物学行为的影响及机制初探  

Effect ofγ-ray irradiation on alveolar epithelial cells and a preliminary study on the mechanism

作　　者：张潇 叶雨萌 李杨 ZHANG Xiao;YE Yumeng;LI Yang(Academy of Mililary Medical Science,Beijing Institute of Radiation Medicine,Beijing 100036,China;Zhengzhou University,Academy of Medical Sciences,Zhengzhou 450000,China)

机构地区：[1]军事科学院军事医学研究院辐射医学研究所,北京100036 [2]郑州大学医学科学院,郑州450000

出　　处：《中国体视学与图像分析》2021年第4期332-338,共7页Chinese Journal of Stereology and Image Analysis

基　　金：北京市自然科学基金面上项目(7132149)。

摘　　要：目的研究γ射线照射对肺泡上皮细胞生物学行为的影响并初步探讨其机制。方法离体培养肺泡上皮细胞A549,分为对照组和照射组,采用5Gy60Coγ射线照射,于照射后的30 min、1 h、3 h、6 h、12 h、24 h及72 h应用MTT法检测细胞增殖活力。于照射后的30 min、1 h、3 h、6 h、12 h应用AO/EB染色法、γH2AX的免疫荧光染色及免疫印迹法细胞凋亡、DNA损伤、AIF和PARP等的表达;并于照射前应用PARP的抑制剂3-AB处理细胞24 h后进行照射,于照射后的30 min、1 h、3 h、6 h、12 h及24 h采用免疫印迹法检测AIF的表达。结果5Gyγ射线照射后3 h、6 h、12 h和72 h,A549细胞照射组增殖显著抑制,且照射后30 min、1 h、3 h A549细胞出现DNA损伤和凋亡,照射后30 min PARP表达升高;应用PARP的特异性抑制剂3-AB显著抑制照射导致AEC的AIF表达增高。结论γ射线照射导致A549细胞发生DNA损伤和凋亡,PARP在该过程中发挥调控作用。Objective To investigate the effect ofγ-ray irradiation on the biological behavior of alveolar epithelial cells and the mechanism.Methods Alveolar epithelial cells(A549)were irradiated with 5 Gy60 Coγ-ray.MTT,AO/EB staining,γH2AX immunofluorescence staining and western blotting were used to detect cell proliferation,cell apoptosis,DNA damage and the expression of AIF and PARP.Then A549 cells were treated with 3-AB,a specific inhibitor of PARP,and the expression of AIF was detected by western blotting at 30 min,1 h,3 h,6 h,12 h and 24 h after irradiation.Results 5 Gyγ-ray irradiation inhibited the proliferation of A549 cells,resulting in DNA damage and apoptosis of A549 cells.The expression of PARP and cleaved PARP in A549 cells increased after 5Gyγ-ray irradiation.Furthermore,3-AB significantly inhibited the increase of AIF in A549 cells caused by irradiation.Conclusionsγ-ray irradiation induced DNA damage and apoptosis of A549 cells,while the activation of PARP might play a regulatory role in this process.

关 键 词：Γ射线 肺泡上皮细胞 DNA损伤 凋亡 

分 类 号：R818[医药卫生—放射医学]