罗平晾晒烟的化学成分研究  

Study on the chemical composition of the sun/air-cured tobacco from Luoping

作　　者：魏敏 程林 祁艳艳 王芳 陈柯瑾 崔艺璇 李干鹏 WEI Min;CHENG Lin;QI Yan-yan;WANG Fang;CHEN Ke-jin;CUI Yi-xuan;LI Gan-peng(Key Laboratory of Chemistry in Ethnic Medicinal Resources,State Ethnic Affairs Commission and Ministry of Education,School of Ethnic Medicine,Yunnan Minzu University,Kunming 650500,China)

机构地区：[1]云南民族大学民族医药学院民族药资源化学国家民委-教育部重点实验室,云南昆明650500

出　　处：《云南民族大学学报（自然科学版）》2022年第2期146-151,共6页Journal of Yunnan Minzu University:Natural Sciences Edition

基　　金：国家自然科学基金(31860100).

摘　　要：采用正相硅胶柱色谱、反相C_(18)柱色谱、Sephadex LH-20凝胶柱色谱及半制备型高效液相等方法从罗平晾晒烟(Nicotiana tabacum.)95%乙醇提取物中分离得到12个化合物.通过现代波谱学技术鉴定化合物的结构分别(1)Cis-abienol,(2)(-)-α-tocospirone,(3)α-tocospiros B,(4)α-tocospiros A,(5)(1S,2E,4-R,6E,8R,11S)-8,11-epoxy-2,6,12(20)-cembratrien-4ol,(6)(1S,2E,4R,6E,8S,11E)-2,6,11-cembratrien-4,8-diol,(7)(1S,2E,4R,7S)-11,12-epoxy-2,8(19)-cembradiene-4,7-diol,(8)Nicotabin A,(9)Stigmasterol,(10)Scopolin,(11)Indole-3-carboxylic acid,(12)α,β-dipyridyl,并对它们进行体外抗HeLa肿瘤活性初步筛选,发现化合物10和12对HeLa细胞增殖具有抑制作用,抑制率分别为20.38%和15.05%.Twelve compounds were isolated from the 95%ethanol extract of the sun/air-cured tobacco of Luoping by normal phase silica gel,C_(18),sephadex LH-20 and semi-preparative high-performance liquid phase(HPLC).Their structures were identified by modern spectroscopic techniques as(1)Cis-abienol,(2)(-)-α-tocospirone,(3)α-tocospiros B,(4)α-tocospiros A,(5)(1S,2E,4-R,6E,8R,11S)-8,11-epoxy-2,6,12(20)-cembratrien-4ol,(6)(1S,2E,4R,6E,8S,11E)-2,6,11-cembratrien-4,8-diols,(7)(1S,2E,4R,7S)-11,12-epoxy-2,8(19)-cembradiene-4,7-diol,(8)Nicotabin A,(9)Stigmasterol,(10)Scopolin,(11)Indole-3-carboxylic acid,(12)α,β-dipyridyl.In addition,compounds 10 and 12 had inhibitory effects on the proliferation of HeLa cells at the concentration of 50μmol/L with inhibitory rates of 20.38%and 15.05%,respectively.

关 键 词：晾晒烟 化学成分 萜类 抗肿瘤活性 

分 类 号：R932[医药卫生—生药学]