海南岛橡胶林土壤真菌群落多样性的季节变化特征及其驱动因子分析  

Seasonal Changes and Driving Factors of Soil Fungal Diversity in the Rubber Plantations in Hainan Island

作　　者：魏亚情 吴志祥[1,2] 杨川 兰国玉[1,2] WEI Ya-qing;WU Zhi-xiang;YANG Chuan;LAN Guo-yu(Rubber Research Institute,Chinese Academy of Tropical Agricultural Sciences,Haikou 571101,Hainan,China;Danzhou Investigation & Experiment Station of Tropical Crops,Ministry of Agriculture,Danzhou 571737,Hainan,China;College of Environment and Plant Protection,Hainan University,Haikou 570228,Hainan,China)

机构地区：[1]中国热带农业科学院橡胶研究所,海南海口571101 [2]农业农村部儋州热带作物科学观测试验站,海南儋州571737 [3]海南大学生态与环境学院,海南海口570228

出　　处：《西北林学院学报》2022年第2期37-42,89,共7页Journal of Northwest Forestry University

基　　金：海南省重点研发项目社会发展方向项目(ZDYF2019145);国家自然科学基金(31770661);海南省自然科学基金高层次人才(320RC733);海南省研究生创新科研课题(Qhyb2021-03)。

摘　　要：探究海南岛橡胶林土壤真菌群落多样性的季节变化特征及其环境驱动因子,为天然橡胶产业的可持续发展提供科学依据。以海南岛橡胶林旱季和雨季的土壤真菌为研究对象,使用Illumina Miseq高通量测序法,对土壤真菌的α、β多样性和群落结构组成及与环境因子的关系进行分析。结果表明,1)海南岛橡胶林共检测到真菌9门、37纲、115目、289科、818属,优势菌门依次为子囊菌门(Ascomycota)、担子菌门(Basidiomycota)和接合菌门(Zygomycota);2)橡胶林土壤真菌雨季土壤真菌丰富度和多样性均高于旱季,基于Bray-Curtis距离的PCoA分析,不同季节土壤真菌群落β多样性差异显著(ANOSIM:R=0.051,P=0.001),PERMANOVA分析结果表明,季节变化解释了真菌群落组成差异的1.7%(R^(2)=0.017,P=0.001);3)真菌α多样性与pH(R^(2)=0.19,P<0.001)、温度(R^(2)=0.14,P<0.001)、全N(TN:R^(2)=0.13,P<0.001)呈显著正相关。温度、全N、有机质、降雨量和pH分别解释了真菌群落组成的8.90%、5.42%、4.73%、4.32%、4.29%。橡胶林土壤真菌群落存在显著的旱雨季变化,pH、温度、全N是影响土壤真菌群落多样性的主要环境因子。The goals of the study were to investigate the changes of fungal composition and diversity across seasons and the driving factors of the rubber plantations in Hainan Island,the results of this study can provide scientific basis for the sustainable development of natural rubber industry.Using Illumina MiSeq high-throughput sequencing method,this study analyzed theαandβdiversity,community structure and the relationship with environmental factors of soil fungi in the rubber plantations in Hainan Island during the dry and rainy seasons.The results showed that 1)Nine phyla,37 classes,115 orders,289 families and 818 genera of fungi were detected in the 130 soil samples.The main dominant fungi phylum across all samples were Ascomycota,Basidiomycota and Zygomycota.2)Theαdiversity index showed that the soil fungal richness and diversity were higher in rainy season than in dry season.The PcoA analysis based on Bray-Curtis distance showed that there was significant difference in theβ-diversity of soil fungal communities in different seasons(ANOSIM:R=0.051,P=0.001).The PERMANOVA analysis showed that seasonal changes explained 1.7%of fungal variance(R^(2)=0.017,P=0.001).3)The results of linear analysis of fungal diversity and environmental factors showed that soil pH(R^(2)=0.19,P<0.001),temperature(R^(2)=0.14,P<0.001)and total nitrogen(TN:R^(2)=0.13,P<0.001)were positively correlated with fungalαdiversity.The RDA results showed that temperature,total nitrogen,soil organic matter,precipitation and pH explained 8.90%5.42%,4.73%,4.32%,and 4.29%of the variations in fungal communities,respectively.The soil fungal community had a significantly change between rainy and dry seasons,pH,temperature and total nitrogen were the main driving factors of soil fungal community diversity in rubber plantation.

关 键 词：橡胶林 真菌群落 多样性 季节变化 

分 类 号：S718.45[农业科学—林学]