共载紫杉醇与胡椒碱的固体脂质纳米粒的体外药物释放  

In vitro release of paclitaxel co-loading with piperine from the solid lipid nanoparticles

作　　者：田思宇 韩新宇 任金妹 丛卉婧 崔欣冉 唐景玲[1] TIAN Si-yu;HAN Xin-yu;REN Jin-mei;CONG Hui-jing;CUI Xin-ran;TANG Jing-ling(College of Pharmacy,Harbin Medical University,Harbin 150086;Qingpu Branch of Zhongshan Hospital Affiliated to Fudan University,Shanghai 201700)

机构地区：[1]哈尔滨医科大学药学院,哈尔滨150086 [2]复旦大学附属中山医院青浦分院,上海201700

出　　处：《中南药学》2022年第3期557-560,共4页Central South Pharmacy

基　　金：黑龙江省自然科学基金项目(No.H_(2)018013);哈尔滨医科大学药学院杰出人才基金项目(No.2019-JQ-03);上海市青浦区科技发展基金项目(No.QKY2019-18);复旦大学附属中山医院青浦分院院级课题(No.QYP2020-03)。

摘　　要：目的制备共载紫杉醇与胡椒碱的固体脂质纳米粒(PP-SLN),测定其粒径及Zeta电位并评价其体外释放行为。方法采用溶剂挥发-高压均质法制备PP-SLN,建立HPLC法测定紫杉醇体外释放的方法学,并采用正向动态膜透析法对PP-SLN体外药物释放进行评价。结果PP-SLN的粒径为(53.72±1.43)nm,Zeta电位为(-19.4±3.31)mV。PP-SLN中的紫杉醇于48 h的累积释放量约为76%,对照制剂(PTX混悬液)中的紫杉醇48 h时累积释放量约为30%。结论本试验制备的PP-SLN粒径小,粒度分布均匀,且可提高紫杉醇的体外释放量。Objective To prepare solid lipid nanoparticles co-loaded with paclitaxel and piperine(PP-SLN),measure their particle sizes and Zeta potential,and evaluate their in vitro release.Methods Solvent volatilization-high pressure homogenization method was used to prepare the PP-SLN.The HPLC method was established to determine the in vitro release of paclitaxel,and the forward dynamic membrane dialysis was used to evaluate the in vitro drug release.Results The diameter of the PP-SLN was(53.72±1.43)nm,and the Zeta potential was(-19.4±3.31)mV.The cumulative release of paclitaxel in the PP-SLN at 48 h was about 76%,while that in the control formulation(PTX suspension)was about 30%.Conclusion The PP-SLNs prepared in this experiment have small particle size and uniform distribution,which increase the in vitro release of paclitaxel.

关 键 词：紫杉醇 胡椒碱 固体脂质纳米粒 体外释放 

分 类 号：R283[医药卫生—中药学]