金茵利胆胶囊的高效液相色谱指纹图谱及其抗氧化作用的灰色关联分析  

Grey correlation analysis of HPLC fingerprint of Jinyin Lidan capsules and their antioxidant in vitro

作　　者：曹凡 陈琳 宋忠兴 唐志书 倪健 CAO Fan;CHEN Lin;SONG Zhong-xing;TANG Zhi-shu;NI Jian(Shaanxi University of Traditional Chinese Medicine,Xianyang Shaanxi 712000;Shaanxi Collaborative Innovation Center for the Industrialization of Traditional Chinese Medicine Resources,State Key Laboratory of Research and Development of Characteristic Qin Medicine Resources(Cultivation),Shaanxi Innovative Drug Research Center,Xianyang Shaanxi 712083;China Academy of Chinese Medical Sciences,Beijing 100700;Beijing Academy of Chinese Medicine,Beijing University of Chinese Medicine,Beijing 100029)

机构地区：[1]陕西中医药大学,陕西咸阳712000 [2]陕西省中药资源产业化协同创新中心,秦药特色资源研究开发国家重点实验室(培育),陕西省创新药物研究中心,陕西咸阳712083 [3]中国中医科学院,北京100700 [4]北京中医药大学北京中医药研究院,北京100029

出　　处：《中南药学》2022年第3期574-579,共6页Central South Pharmacy

基　　金：国家自然科学基金青年科学基金项目(No.81904047);陕西省重点研发计划项目(No.2019SF-285);中药大品种品牌价值提升示范研究(No.2020190025);现代农业产业技术体系建设专项资金(No.CARS-21);咸阳市2020重大科技专项(No.2020K01-20)。

摘　　要：目的建立金茵利胆胶囊高效液相色谱指纹图谱并进行其与抗氧化作用的灰色关联分析。方法采用中药色谱指纹图谱相似度评价系统(2012版)对15批样品进行相似度分析;对均匀设计的N0~N7样品的共有峰进行体外DPPH和ABTS清除自由基的抗氧化活性测定,以清除率为药效指标,进行灰色关联分析。结果15批次样品指纹图谱叠加出23个共有峰,相似度均大于0.900。经对照品化学指认确定出7个共有峰(新绿原酸、绿原酸、隐绿原酸、1,3-二咖啡酰奎宁酸、对羟基苯乙酮、柚皮苷和新橙皮苷)。谱效关系研究结果表明峰12、峰14(绿原酸)、峰23(新橙皮苷)、峰10、峰17(对羟基苯乙酮)、峰8、峰13(隐绿原酸)和峰9(新绿原酸)抗氧化活性贡献度较强。结论本研究对金茵利胆胶囊谱效关系进行关联分析,为其抗氧化活性成分的筛选提供参考。Objective To conduct grey correlation analysis of the HPLC fingerprint for Jinyin Lidan capsules and their antioxidant.Methods Fifteen batches of samples were analyzed by similarity evaluation system of TCM chromatographic fingerprint(2012 edition)and 7 index components were measured.The free radical scavenging antioxidant activities of DPPH and ABTS of uniformly designed common peaks N0~N7 were determined in vitro.The scavenging rate was used as pharmacodynamic index grey correlation analysis.Results The fingerprint of 15 batches of samples superimposed 23 common peaks,with similarity>0.900.Seven common peaks(neochlorogenic acid,chlorogenic acid,cryptochlorogenic acid,1,3-dicaffeic quinic acid,p-hydroxyacetophenone,naringin and neoperidin)were identified by reference chemicals.Peaks 12,14(chlorogenic acid),23(neohesperidin),10,17(p-hydroxyacetophenone),8,13(cryptochlorogenic acid)and 9(neochlorogenic acid)contributed significantly to the antioxidant activity.Conclusion The correlation analysis of between the spectrum and effect of Jinyin Lidan capsules provides reference for the screening of antioxidant active ingredients of Jinyin Lidan capsules.

关 键 词：金茵利胆胶囊 高效液相色谱 指纹图谱 抗氧化作用 灰色关联分析 

分 类 号：R284[医药卫生—中药学]