高效液相色谱波长切换法同时测定三拗片中5种成分的含量  

Simultaneous determination of 5 components in San’ao tablets by wavelength switching HPLC

作　　者：梁冬牛 郑磊 李娟[2] LIANG Dong-niu;ZHENG Lei;LI Juan(Department of Pharmacy,Shandong Provincial Third Hospital,Ji’nan 250031;Department of Pharmacy,The Second Hospital of Shandong University,Ji’nan 250033)

机构地区：[1]山东省立第三医院药学部,济南250031 [2]山东大学第二医院药学部,济南250033

出　　处：《中南药学》2022年第3期605-609,共5页Central South Pharmacy

基　　金：山东省自然科学基金(No.ZR2017BH014);山东省重点研发计划(No.2018GSF118010)。

摘　　要：目的建立高效液相色谱法同时测定三拗片中盐酸麻黄碱、盐酸伪麻黄碱、苦杏仁苷、甘草苷与甘草酸5种成分的含量。方法样品采用超声波方法进行提取,色谱柱为Capcell pak C_(18)(4.6 mm×250 mm,5μm),流动相为乙腈-0.1%磷酸,梯度洗脱,流速为1.0 mL·min^(-1),检测波长为205 nm(盐酸麻黄碱、盐酸伪麻黄碱和苦杏仁苷)与237 nm(甘草苷和甘草酸),柱温为30℃。结果盐酸麻黄碱、盐酸伪麻黄碱、苦杏仁苷、甘草苷与甘草酸检测的线性范围分别为1.636~81.80 mg·m^(L-1)(r=0.9999)、1.207~60.35 mg·m^(L-1)(r=0.9998)、8.609~430.45 mg·m^(L-1)(r=0.9998)、1.304~65.20 mg·m^(L-1)(r=0.9999)、2.539~126.95 mg·m^(L-1)(r=0.9998),平均回收率分别为98.2%(RSD=1.3%)、101.0%(RSD=2.0%)、102.8%(RSD=1.5%)、102.4%(RSD=1.3%)、97.0%(RSD=1.1%)。结论该方法重复性好、结果准确,为三拗片质量控制提供了含量测定方法。Objective To simultaneously determine the content of ephedrine hydrochloride,pseudoephedrine hydrochloride,amygdalin,liquiritin and glycyrrhizic acid in San’ao tablet by wavelength switching HPLC.Methods Samples were extracted by ultrasonic method.The chromatographic column was Capcell pak C_(18)(4.6 mm×250 mm,5μm)and the acetonitrile-0.1%phosphoric acid was used as the mobile phase with gradient elution.The flow rate was 1.0 mL·min^(-1).The detection wavelength was 205 nm for ephedrine hydrochloride,pseudoephedrine hydrochloride and amygdalin,and 237 nm for liquiritin and glycyrrhizic acid.The column temperature was 30℃.Results The linearity was 1.636~81.80 mg·m^(L-1) for ephedrine hydrochloride(r=0.9999),1.207~60.35 mg·m^(L-1) for pseudoephedrine hydrochloride(r=0.9998),8.609~430.45 mg·m^(L-1) for amygdalin(r=0.9998),1.304~65.20 mg·m^(L-1) for liquiritin(r=0.9999),and 2.539~126.95 mg·m^(L-1) for glycyrrhizic acid(r=0.9998),respectively.The average recoveries were 98.2%(RSD=1.3%),101.0%(RSD=2.0%),102.8%(RSD=1.5%),102.4%(RSD=1.3%),and 97.0%(RSD=1.1%),respectively.Conclusion The method is simple and accurate,and can be used for simultaneous determination of 5 components in San’ao tablets.

关 键 词：三拗片 高效液相色谱法 含量测定 

分 类 号：R917[医药卫生—药物分析学] R286[医药卫生—药学]