补肾活血方对子痫前期模型大鼠血栓前状态中凝血功能及TFPI-2的影响研究  

Effects of Bushen Huoxue Decoction on the Coagulation Function and TFPI-2 in the Pre-thrombotic State Rats with Preeclampsia

作　　者：蔡晓晓 袁宁霞[1] 张小菜 裴巧丽[1] 王丹[1] 王甜 朱丽红[1] Cai Xiaoxiao;Yuan Ningxia;Zhang Xiaocai;Pei Qiaoli;Wang Dan;Wang Tian;Zhu Lihong(The Second Affiliated Hospital of Shaanxi University of Chinese Medicine,Shaanxi,Xianyang 112000,China)

机构地区：[1]陕西中医药大学第二附属医院,陕西咸阳712000

出　　处：《中国中医急症》2022年第3期408-411,420,共5页Journal of Emergency in Traditional Chinese Medicine

基　　金：陕西省中医药管理局中医药科研课题(LCMS015);陕西中医药大学第二附属医院学科创新团队(2020XKTD-A05)。

摘　　要：目的本研究通过建立子痫前期大鼠模型,探讨不同剂量补肾活血方对子痫前期大鼠模型血栓前状态中血小板、凝血四项的影响,免疫组化法研究TFPI-2在胎盘中的表达。方法选用一氧化氮合成酶抑制剂——左旋亚硝基精氨酸甲酯(L-NAME)作为造模药物,将造模成功的72只大鼠按照随机数字表法分为6组,正常妊娠组、子痫前期组、阿司匹林组、补肾活血方低剂量组、补肾活血方中剂量组、补肾活血方高剂量组,每组12只。分别于妊娠前、妊娠第10、15、20天测量大鼠收缩压;妊娠第10、15、20天检测24 h尿蛋白;孕21 d麻醉后腹主动脉取血检测血小板、凝血四项;取胎盘免疫组化检测组织因子途径抑制物-2(TFPI-2)的表达;将实验结果进行统计分析。结果L-NAME能成功建立子痫前期模型大鼠,使收缩压及24 h尿蛋白升高,血小板计数(PLT)升高,凝血酶原时间(PT)、活化部分凝血活酶时间(APTT)、凝血酶时间(TT)缩短,纤维蛋白原(Fbg)升高(P<0.05);用药后补肾活血方各剂量组均能不同程度降低大鼠收缩压及24 h尿蛋白,降低PLT、Fbg,升高PT、APTT、TT,差异有统计学意义(P<0.05);中剂量组作用明显优于高剂量组、低剂量组及阿司匹林组(P<0.01);子痫前期组胎盘TFPI-2表达高于正常妊娠组,差异有统计学意义(P<0.05),中剂量组胎盘中TFPI-2的表达较子痫前期组明显下降,差异明显具有统计学意义(P<0.01)。结论补肾活血方能不同程度改善孕鼠血小板、凝血指标,减少胎盘微血栓形成,减少TFPI-2在大鼠胎盘组织中的表达。Objective:To study the effect and significance of different dosages of Bushen Huoxue Decoction on platelet,blood coagulation in preeclampsia rat model by establishing preeclampsia rat model,and to study the expression of TFPI-2 in placenta by immunohistochemistry.Methods:L-NAME,an inhibitor of nitric oxide synthase,was used as the model drug.A total of 72 rat models were randomly divided into 6 groups,12 rats in each group,including the normal pregnancy group,preeclampsia group,aspirin group,Bushen Huoxue Decoction lowdose group,Bushen Huoxue Decoction medium dose group,and Bushen Huoxue Decoction high dose group.The systolic blood pressure of rats was measured before pregnancy and on day 10,15 and 20 of pregnancy.The 24 h urinary protein was detected at 10,15 and 20 days of gestation.Blood samples from the abdominal aorta were collected after anesthesia at 21 days of pregnancy to detect four items of platelet and coagulation.The expression of TFPI-2 was detected by immunohistochemistry of placenta.The experimental results were statistically analyzed.Results:L-NAME could successfully establish preeclampsia rat model,increase systolic blood pressure and 24 h urinary protein,increase PLT,PT,APTT,TT,and decrease Fbg(P<0.05).After administration of Bushen Huoxue Decoction,each dose group could reduce the systolic blood pressure and 24 h urinary protein,decrease PLT,Fbg,and increase PT,APTT,TT in different degrees(P<0.05).The effect of medium dose group was significantly better than that of high dose group,low dose group and aspirin group(P<0.01).The expression of TFPI-2 in the placen-ta of the preeclampsia group was higher than that of the normal pregnancy group,and the difference was statistical-ly significant(P<0.05).The expression of TFPI-2 in the placenta of the medium dose group was significantly low-er than that of the model group,and the difference was statistically significant(P<0.01).Conclusion:The experi-mental study proves that Bushen Huoxue Decoction can improve platelet and blood coagulation indexes in p

关 键 词：子痫前期 补肾活血方 凝血功能 TFPI-2 大鼠 

分 类 号：R285.5[医药卫生—中药学]