Development of high-oil maize haploid inducer with a novel phenotyping strategy  

作　　者：Chenxu Liu Jinlong Li Ming Chen Wei Li Yu Zhong Xin Dong Xiaowei Xu Chen Chen Xiaolong Tian Shaojiang Chen 

机构地区：[1]College of Agronomy and Biotechnology,Beijing Key Laboratory of Crop Genetic Improvement,Laboratory of Crop Heterosis and Utilization,Ministry of Education,National,Maize Improvement Center of China,China Agricultural University,Beijing 100193,China [2]Chongqing Academy of Agricultural Sciences,Chongqing 401329,China

出　　处：《The Crop Journal》2022年第2期524-531,共8页作物学报（英文版）

基　　金：supported by the National Key Research and Development Program of China(2016YFD0101200);the China Agriculture Research System(CARS-02);2020 Research Program of Sanya Yazhou Bay Science and Technology City(SKJC-2020-02-03);the National Natural Science Foundation of China(91935303,32001554)。

摘　　要：Doubled haploid(DH) technology is important in modern maize breeding. Haploid inducers determine the efficiency of both haploid induction and identification. It has taken decades to improve the efficiency,haploid induction rate(HIR), from the ~2% of the ancestor haploid inducer, stock6, to the ~10% of modern haploid inducers. Improvement of kernel oil content(KOC) would further enhance haploid identification efficiency. Using molecular marker-assisted selection, in combine with the number of haploids per ear as phenotypic criterion, we developed a new high-oil haploid inducer line, CHOI4, with a mean HIR of 15.8%and mean KOC of 11%. High KOC of CHOI4 can achieve a mean accuracy greater than 90% in identification of haploids of different backgrounds, with reduced false discovery rates and false negative rates in comparison with the previous high-oil haploid inducer line, CHOI3. Comparison of phenotypic selection strategies suggested that the number of haploids per ear can be used as a phenotyping criterion during haploid inducer line development. CHOI4 could further increase the efficiency of large-scale DH breeding programs with lower cost.

关 键 词：Doubled haploid Marker-assisted selection Kernel oil content Haploid identification Haploids per ear 

分 类 号：S513[农业科学—作物学]