Identification of genes involved in the formation of soluble dietary fiber in winter rye grain and their expression in cultivars with different viscosities of wholemeal water extract  

作　　者：Liudmila V.Kozlova Alsu R.Nazipova Oleg V.Gorshkov Liliya F.Gilmullina Olga V.Sautkina Natalia V.Petrova Oksana I.Trofimova Sergey N.Ponomarev Mira L Ponomareva Tatyana A.Gorshkova 

机构地区：[1]Kazan Institute of Biochemistry and Biophysics,Federal Research Center,Kazan Scientific Center of the Russian Academy of Sciences,420111 Kazan,Russia [2]Tatar Scientific Research Institute of Agriculture,Federal Research Center,Kazan Scientific Center of the Russian Academy of Sciences,420059 Kazan,Russia

出　　处：《The Crop Journal》2022年第2期532-549,共18页作物学报（英文版）

基　　金：supported by Russian Foundation for Basic Research with project number of i_m_17-29-08023(Liudmila V.Kozlova,Alsu R.Nazipova,Oleg V.Gorshkov,Liliya F.Gilmullina,Natalia V.Petrova,Sergey N.Ponomarev,Mira L.Ponomareva,Tatyana A.Gorshkova);Part of work(immunodot binding assay,Olga V.Sautkina;monosaccharide analysis,Oksana I.Trofimova;viscosity of water extract determination,Liliya F.Gilmullina)was performed with financial support from the government assignment for FRC Kazan Scientific Center of RAS。

摘　　要：The grain of rye(Secale cereale L.) used for baking contains a large amount of non-starch polysaccharides,making it an excellent component of functional foods. But rye grain intended for alcohol production and forage use should have a reduced content of these polysaccharides. A comprehensive parameter that can predict the best field of application for winter rye grain is the viscosity of its wholemeal water extract.However, our understanding of the genetic background underlying this key trait and associated features of rye grain is poor. By analyzing six Russian winter rye cultivars, we identified the most contrasting forms and characterized the peculiarities of their water-soluble carbohydrates capable of influencing the viscosity of water extracts. Then, using phylogenetic and transcriptomic analyses, we identified in the rye genome many genes encoding putative glycosyltransferases and glycosylhydrolases responsible for the synthesis and degradation of arabinoxylans, mixed-linkage glucans, cellulose, and some other polysaccharides. We determined the dynamics of m RNA abundance for these genes at three stages of kernel development. Comparisons of gene expression levels in two contrasting cultivars revealed specific members of multigene families that may serve as promising targets for manipulating non-starch polysaccharide content in rye grain. High-viscosity cultivars were characterized by up-regulation of many glycosyltransferases involved in the biosynthesis of arabinoxylans and other cell-wall polysaccharides,whereas low-viscosity cultivars showed up-regulation of several genes encoding polysaccharidedegrading enzymes.

关 键 词：Rye(Secale cereale) Kernel development ARABINOXYLAN Mixed-linkage glucan Viscosity 

分 类 号：S512.5[农业科学—作物学]