温莪术药渣再提取工艺及其美白活性研究  

Study on the re-extraction process and whitening activity of Curcuma wenyujin dregs

作　　者：夏禹 张文珍 黄真[1] 仇凤梅[1] 钟晓明[1] XIA Yu;ZHANG Wen-zhen;HUANG Zhen;QIU Feng-mei;ZHONG Xiao-ming(College of Pharmaceutical Science,Zhejiang Chinese Medical University,Hangzhou 310053,China)

机构地区：[1]浙江中医药大学药学院,杭州310053

出　　处：《天然产物研究与开发》2022年第3期457-464,共8页Natural Product Research and Development

基　　金：株式会社高丝研究所横向课题(2016-HT-146);国家级大学生创新创业训练项目(202110344003X)。

摘　　要：以活性成分总姜黄素得率为指标,响应面法优化温莪术药渣的最佳提取工艺,采用DPPH法和ABTS法评价温莪术药渣提取物的抗氧化活性,并考察其对小鼠黑色素瘤B16细胞存活率、黑色素合成及其细胞内酪氨酸酶活性的影响。实验结果表明,温莪术药渣提取物的最佳工艺条件为乙醇浓度82%,超声时间55 min,液料比22 mL/g,总姜黄素平均提取得率为6.38 mg/g;此外,温莪术药渣提取物对DPPH、ABTS自由基具有一定的清除作用,IC_(50)值分别为24.99、49.72μg/mL;并且能有效抑制B16细胞黑色素合成及酪氨酸酶活性,与正常组相比均具有显著性差异(P<0.05)。本研究为温莪术提油后药渣二次开发利用提供了依据。The total curcumin yield was used as an index for optimizing the conditions of the extraction of Curcuma wenyujin dregs with response surface methodology.The antioxidant activity of the extracts was determined by the DPPH and ABTS free radical inhibition methods,and the influence of them on survival rate,melanin synthesis and tyrosinase activity in mouse melanoma B16 cells was also investigated.The results showed that the optimal extraction conditions were:ethanol concentration 82%,ultrasonic time 55 min,liquid/solid ratio 22 mL/g.The average extraction yield of total curcumin was 6.38 mg/g.In addition,the extracts from C.wenyujin dregs could scavenge DPPH and ABTS free radicals with IC_(50) values of 24.99μg/mL and 49.72μg/mL,respectively.Further,it can effectively inhibit melanin synthesis and tyrosinase activity of B16 cells,with significant differences compared with normal control(P<0.05).This study provided the basis for the secondary development and utilization on the dregs of C.wenyujin after extracting essential oil.

关 键 词：温莪术 响应面 酪氨酸酶 抗氧化 美白活性 

分 类 号：TQ461[化学工程—制药化工]