薏苡仁提取物对糖尿病肾病大鼠肾功能的作用及机制研究  

Effect and mechanism of Coix seed extract on renal function in diabetic nephropathy rats

作　　者：彭湘[1] 陈丽[1] 薛琳[1] Xiang Peng;Li Chen;Lin Xue(Department of Nephrology,Panzhihua Central Hospital,Panzhihua,Sichuan 617067,China)

机构地区：[1]攀枝花市中心医院肾病内科,四川攀枝花617067

出　　处：《中国现代医学杂志》2022年第3期10-15,共6页China Journal of Modern Medicine

摘　　要：目的探讨薏苡仁提取物(CSE)对糖尿病肾病大鼠肾脏功能的保护作用及相关机制。方法对大鼠腹腔注射小剂量链脲佐菌(STZ)复制糖尿病肾病模型。采取不同的干预措施,将50只大鼠随机分为5组:对照组(ND组)、链脲佐菌模型组(STZ组)、格列齐特治疗组(GLI组)、CSE低剂量组(CSE-L组),CSE高剂量组(CSE-H组)。检测各组大鼠空腹血糖水平、24 h尿蛋白含量、初始体重、最终体重、肾脏重量及肾组织损伤指标。Western blotting法检测肾组织中转化生长因子-β1(TGF-β1)和髓样分化因子88(MyD88)蛋白的相对表达量。结果各组大鼠不同时间的空腹血糖水平差异有统计学意义(P<0.05),6周时血糖低于1周和3周时血糖;各组大鼠的空腹血糖水平差异有统计学意义(P<0.05),STZ组高于ND组(P<0.05),CSE-L组、CSEH组低于STZ组(P<0.05)。各组大鼠空腹血糖水平的变化趋势差异有统计学意义(P<0.05)。各组大鼠24 h尿蛋白含量、初始体重、最终体重比较,差异有统计学意义(P<0.05),STZ组24 h尿蛋白水平高于ND组(P<0.05),初始体重、最终体重低于ND组(P<0.05),CSE-L组、CSE-H组24 h尿蛋白水平低于STZ组(P<0.05),初始体重、最终体重高于STZ组(P<0.05)。各组大鼠肾功能参数比较,差异有统计学意义(P<0.05),STZ组Scr、BUN、TC、HDL-C及IL-6水平高于ND组(P<0.05);CSE-H组Scr、BUN、TG、TC、HDLC、GSH、IL-6水平低于STZ组(P<0.05)。各组大鼠肾组织中TGF-β1和MyD88蛋白相对表达量比较,差异有统计学意义(P<0.05),STZ组肾组织中TGF-β1蛋白相对表达量高于ND组(P<0.05),MyD88蛋白相对表达量低于ND组(P<0.05),CSE-H组肾组织中TGF-β1蛋白相对表达量低于STZ组(P<0.05),MyD88蛋白相对表达量高于STZ组(P<0.05)。结论CSE可通过调节TGF-β1、MyD88抑制糖尿病肾病大鼠肾脏纤维化进程和炎症反应,具有良好的肾脏保护作用。Objective To investigate the protective effect of CSE on renal function in DN rats and its mechanism. Methods Fifty rats were randomly divided into ND group, STZ group, Gli group, CSE-L group, and CSE-H group. Fasting blood glucose level, 24-hour urinary protein content, initial body weight, final body weight,renal mass and renal tissue injury indexes were measured. The expression of MyD88 protein and TGF-β in renal tissue were detected. Results The fasting blood glucose levels of rats in each group were compared at different time points, 6 w was lower than 1 w and 3 w, and the difference was statistically significant(P < 0.05);that in STZ group was higher than in ND group(P < 0.05);that in CSE-L group and CSE-H group were higher than in STZ group. The24 h urine protein content, initial weight, and final weight of the rats in each group were compared with statistical significance(P < 0.05);the urine protein level of the STZ group was higher than that of the ND group, and the initial and final weight were lower than the ND group(P < 0.05). CSE-L group and CSE-H group had lower urine protein levels than STZ group, and initial body weight and final body in CSE-L group and CSE-H group weight were higher than in STZ group(P < 0.05). The comparison of the renal function parameters of rats in each group showed statistically significant differences(P < 0.05);the levels of Scr, BUN, TC, HDL-C, and IL-6 in the STZ group were higher than those in the ND group(P < 0.05);the levels of Scr, BUN, TG, TC, HDL-C, GSH and IL-6 in CSE-H group were lower than those in STZ group(P < 0.05). The expression of TGF-β1 and MyD88 in the kidney tissue of rats in each group was compared, and the difference was statistically significant(P < 0.05). The expression level of TGF-β1 protein in the kidney tissue of the STZ group was higher than that of the ND group(P < 0.05);the protein expression level of MyD88 was lower than that of the ND group(P < 0.05);the TGF-β1 protein expression level in the kidney tissue of the CSE-H group was lower th

关 键 词：糖尿病肾病 薏苡仁提取物 转化生长因子-Β1 髓样分化因子88 

分 类 号：R587.2[医药卫生—内分泌]