化学预处理与傅里叶变换红外光谱相结合分析棉花纤维细胞壁组分  

Analysis of Components of Cotton Fiber Cell Wall by Chemical Pretreatment and Fourier Transform Infrared Spectroscopy(FTIR)

作　　者：胡文冉[1] 杨洋[1] 李波[1] 郝晓燕[1] 赵准 邵武奎 黄全生[1] HU Wenran;YANG Yang;LI Bo;HAO Xiaoyan;ZHAO Zhun;SHAO Wukui;HUANG Quansheng(Xinjiang Key Laboratory of Crop Biotechnology/Research Institute of Nuclear Technology and Biotechnology, Xinjiang Academy of Agricultural Sciences,Urumqi 830091, China)

机构地区：[1]新疆农业科学院核技术生物技术研究所/新疆农作物生物技术重点实验室,乌鲁木齐830091

出　　处：《新疆农业科学》2022年第2期261-268,共8页Xinjiang Agricultural Sciences

基　　金：新疆农作物生物技术重点实验室开放课题(XJYS0302-2020-02);国家转基因专项(2011ZX08005-005);新疆重点研发计划(2016B01001-1)。

摘　　要：【目的】研究能够与傅里叶变换红外光谱(FTIR)相结合的分析棉花纤维细胞壁组分的化学预处理方法,使棉花纤维细胞壁断裂破碎但不造成其组分结构破坏,为实现应用FTIR分析细胞壁的真实结构组分提供参考。【方法】采用化学试剂巯基乙酸和盐酸预处理棉花纤维,用去离子水清洗游离的化学试剂,再通过FTIR化学预处理后的棉花纤维细胞壁。利用木质化程度和硬度较高的樟子松枝条进行相同处理作为对照,观察分析。【结果】采用化学试剂巯基乙酸和盐酸预处理棉花纤维,纤维在其与巯基乙酸中的巯基结合的部位被打碎,可以有效破碎棉花纤维细胞壁,克服了纤维样品难以磨碎的问题,再用去离子水清洗游离的化学试剂,通过FTIR观察化学预处理后的棉花纤维细胞壁,发现化学处理过的棉花纤维在1800~1650 cm^(-1)的波谱区间内显著高于原始切碎棉花纤维,在1800~1550 cm^(-1)的波谱区间内,化学处理棉花纤维和切碎纤维间存在极显著差异,在1320~1200 cm^(-1)波谱内,处理棉花纤维的FTIR光谱吸光度值显著高于切碎棉花纤维。【结论】化学试剂巯基乙酸和盐酸预处理棉花纤维,再与FTIR相结合可以正确评价棉花纤维细胞壁组分。【Objective】To provide a chemical pretreatment method which can be combined with fourier transform infrared spectroscopy(FTIR)to observe the cell wall components of cotton fiber.In the method,the cell wall of cotton fiber can be broken without causing structural damage and the real structural components of cell wall can be analyzed by FTIR.【Method】Cotton fiber was pretreated with thioglycolic acid and hydrochloric acid,and the free chemical reagent was washed with deionized water.The cell wall of cotton fiber after chemical pretreatment was observed by FTIR.Using the same treatment of the shoots of Pinus sylvestris L.var.mongolica Litv.with high lignification and hardness as control to verify the treatment method.【Result】Cotton fiber was broken at the site where it binds to the sulfhydryl group in thioglycolic acid,which could effectively break the cell wall of cotton fiber and overcome the problem that it was difficult to grind the fiber sample.Then the free chemical reagents were washed with deionized water.The cell wall of cotton fiber after chemical pretreatment was observed by FTIR.It was found that the chemically treated cotton fiber was significantly higher than the original chopped cotton fiber in the spectral range of 1,800-1,650 cm^(-1).In the spectral range of 1,800-1,550 cm^(-1),there was a very significant difference between chemically treated cotton fiber and chopped fiber,in the spectral range of 1,320-1,200 cm^(-1),The FTIR absorbance of treated cotton fiber was significantly higher than that of chopped cotton fiber.【Conclusion】The cell wall components of cotton fiber can be correctly evaluated by pretreatment of cotton fiber with thioglycolic acid and hydrochloric acid and then combined with FTIR.

关 键 词：棉花 细胞壁 傅里叶变换红外光谱 化学预处理 

分 类 号：S562[农业科学—作物学]