甘薯蔓割病拮抗菌株KC2-8的筛选、鉴定及紫外诱变  

Screening, identification and UV mutagenesis of antagonistic strain against KC2-8 sweetpotato stem rot

作　　者：张静珍 靳晓杰 王连军[1] 柴沙沙[1] 龙同[3] 杨园园[1] 程贤亮[1] 杨新笋[1] 雷剑[1] Zhang Jingzhen;Jin Xiaojie;Wang Lianjun;Chai Shasha;Long Tong;Yang Yuanyuan;Cheng Xianliang;Yang Xinsun;Lei Jian(Hubei Academy of Agricultural Sciences«Institute of Food Corp/Hubei Key Laboratory of Food Crops Germplasm&Genetic Improvement/Hubei Sweet Potato Engineering&Technology Research Centre,Wuhan 430064,Hubei,China;College of Agriculture,Yangtze University,Jingzhou 434025,Hubei,China;National Biopesticide Engineering Research Centre/Hubei Biopesticide Engineering Research Centre/Hubei Academy of Agricultural Sciences,Wuhan 430064,Hubei,China)

机构地区：[1]湖北省农业科学院粮食作物研究所/粮食作物种质创新与遗传改良湖北省重点实验室/湖北省甘薯工程技术研究中心,湖北武汉430064 [2]长江大学农学院,湖北荆州434025 [3]国家生物农药工程技术研究中心/湖北省生物农药工程研究中心/湖北省农业科学院,湖北武汉430064

出　　处：《江苏师范大学学报（自然科学版）》2022年第1期25-29,F0002,共6页Journal of Jiangsu Normal University：Natural Science Edition

基　　金：国家重点研发计划(2019YFD1001303);湖北省农业科技创新中心湖北省农科院领军人才培养计划(L2018005);财政部和农业农村部:国家现代农业产业技术体系资助;湖北省农业科技成果转化项目(2020BBB027);湖北省重点研发计划项目(2020BHB024);湖北省农业科学院特色学科项目;湖北省农业科技创新中心项目。

摘　　要：为了筛选对甘薯蔓割病拮抗活性较好的菌株,采用平板对峙法对3株细菌菌株进行抑菌活性评估,并对新筛选的高效抑制甘薯蔓割病的菌株进行分子鉴定和紫外诱变研究.结果显示,细菌菌株KC2-8对尖孢镰刀杆菌有较强的抑制作用.通过16S rDNA序列检测后进行同源性分析,将该菌株鉴定为特基拉芽孢杆菌(Bacillus tequilensis).对KC2-8菌株进行紫外诱变显示,最佳诱变时间为20 s,进一步涂布筛选获得菌落直径大、生长迅速的6株稳定高产单菌株.综合分析表明,特基拉芽孢杆菌KC2-8对甘薯蔓割病菌具有良好抑制作用.To screen the bacteria strain with high antagonistic activity against sweetpotato stem rot,the inhibitory efficiencies of 3 strains of bacteria were determined by the methods of plate confrontation,as well as molecular identification and UV mutagenesis of the most efficient antagonistic strains were studied.The results reviewed that the culture filtrate of strain KC2-8 had the strongest inhibitory effect on sweetpotato stem rot.Based on 16 S rDNA sequence analysis,the strain KC2-8 was identified as Bacillus tequilensis.UV mutagenesis results showed that the optimal irradiation time of original stain of KC2-8 was 20 s.Through screening,6 stable mutant strain with high yield were obtain.This results indicated that Bacillus tequilensis KC2-8 had significant inhibitory effect on sweetpotato stem rot.

关 键 词：甘薯蔓割病 尖孢镰刀杆菌 特基拉芽孢杆菌 紫外诱变 抑制作用 

分 类 号：S531[农业科学—作物学]