腐植酸对李氏禾-红壤处理系统中铬赋存形态、微生物群落及酶活性的影响  

EFFECTS OF HUMIC ACID ON CHROMIUM SPECIATION, MICROBIAL COMMUNITIES AND ENZYME ACTIVITIES IN RED SOIL TREATMENT SYSTEM PLANTED WITH LEERSIA HEXANDRA SWARTZ

作　　者：郭越宏 王建生 张学洪[1,2] 张杏锋[1,2] 高波[3,4] GUO Yue-hong;WANG Jian-sheng;ZHANG Xue-hong;ZHANG Xing-feng;GAO Bo(Guangxi Key Laboratory of Environmental Pollution Control Theory and Technology,Guilin University of Technology,Guilin 541004,China;Collaborative Innovation Center for Water Pollution Control and Water Safety in Karst Area,Guilin University of Technology,Guilin 541004,China;College of Plant and Ecological Engineering,Guilin University of Technology,Guilin 541004,China;College of Tourism&Landscape Architecture,Guilin University of Technology,Guilin 541004,China)

机构地区：[1]桂林理工大学广西环境污染控制理论与技术重点实验室,广西桂林541004 [2]桂林理工大学岩溶地区水污染控制与用水安全保障协同创新中心,广西桂林541004 [3]桂林理工大学植物与生态工程学院,广西桂林541004 [4]桂林理工大学旅游与风景园林学院,广西桂林541004

出　　处：《环境工程》2021年第12期234-242,共9页Environmental Engineering

基　　金：广西自然科学基金(2018GXNSFAA138045);广西科技重大专项(桂科AA17204047);国家重点研发计划项目(2016YFD0800800);广西高等学校高水平创新团队及卓越学者计划项目(桂财教函[2018]319号);广西矿冶与环境科学实验中心(KH2012ZD004)。

摘　　要：研究了不施加和施加10g/kg腐植酸后,李氏禾(Leersia hexandra Swartz)-红壤处理系统中基质和李氏禾体内铬赋存形态、微生物群落数量及酶活性的变化。结果表明:施加腐植酸后,基质中以残渣态铬为主;李氏禾茎叶中以残渣态铬为主,根中则以盐酸提取态铬为主。腐植酸能显著促进细菌、真菌和放线菌生长繁殖,3大菌群数量最大值分别为6.76×10^(7),4.48×10^(7),7.71×10^(7)CFU/g。酶活性分析表明,施加腐植酸有利于提高蔗糖酶、碱性磷酸酶活性,降低过氧化氢酶和多酚氧化酶酶活性。相关性分析表明,多酚氧化酶和过氧化氢酶活性均与细菌数量和腐植酸浓度呈负相关,细菌、真菌和放线菌数量与腐植酸浓度呈正相关。3大菌群数量均与基质残渣态铬含量呈正相关。蔗糖酶与基质和李氏禾体内铬形态含量均呈负相关;多酚氧化酶活性与李氏禾体内大部分铬形态含量呈正相关。The changes of chromium speciation, microbial communities and enzyme activities in red soil treatment system planted with Leersia hexandra Swartz with or without 10 g/kg humic acid were studied. The results showed that residual fraction was dominant in the substrate after humic acid added. The residual fraction was dominant in the stem and leaves while the hydrochloric acid fraction was dominant in the root. The number of bacteria, fungi and actinomycete could be significantly promoted by humic acid, and the maximum number of the three microbial groups was 6.76×10^(7), 4.48×10^(7), 7.71×10^(7)CFU/g. The analysis of enzyme activities showed that humic acid increased the activity of invertase and alkaline phosphatase and decreased the activity of catalase and polyphenol oxidase. The correlation analysis showed that the activities of polyphenol oxidase and catalase were negatively correlated with the number of bacteria and the concentration of humic acid. The number of bacteria, fungi and actinomycetes was positively correlated with the concentration of humic acid. The number of bacteria, fungi and actinomyces were positively correlated with the content of chromium in the substrate residual fraction. A negative correlation existed between invertase and the content of chromium fractions in substrate and in Leersia hexandra Swartz. There was a positive correlation between the activity of polyphenol oxidase and most chromium fractions in Leersia hexandra Swartz.

关 键 词：腐植酸 李氏禾 铬形态 微生物群落 土壤酶活 

分 类 号：X53[环境科学与工程—环境工程]