黄瓜纤维素合成酶CESA家族基因鉴定及非生物胁迫响应分析  

Identification and Abiotic Stress Response Analysis of CESA Family Genes in Cucumber(Cucumis sativus L.)

作　　者：康忱 赵雪芳 王鹏[1] 李亚栋[1] 田哲娟 吴志明[1] KANG Chen;ZHAO Xuefang;WANG Peng;LI Yadong;TIAN Zhejuan;WU Zhiming(Institute of Cash Crops,Hebei Academy of Agricultural and Forestry Sciences,Shijiazhuang 050051,Hebei,China)

机构地区：[1]河北省农林科学院经济作物研究所,河北石家庄050051

出　　处：《中国蔬菜》2022年第3期29-41,共13页China Vegetables

基　　金：河北省省级科技计划资助项目(19226323D);河北省农林科学院科技创新人才队伍建设项目(C21R0801)。

摘　　要：纤维素合成酶(cellulose synthase,CESA)是纤维素合成过程中重要的酶类,在细胞壁建成和植物生长发育过程中起着非常重要的作用。本试验从黄瓜品系9930全基因组数据中鉴定得到35个CESA基因家族成员,系统进化分析将家族成员分成6个group。亚细胞结果显示,大部分成员定位于质膜上,少部分定位在高尔基体上。物种间CESA家族基因共线性分析显示,拟南芥和番茄的CESA基因家族与黄瓜CESA基因家族分别有15个和12个同源基因对,西瓜和甜瓜CESA基因家族与黄瓜CESA基因家族分别有19个和20个同源基因对。顺式作用元件分析表明,CsCESA家族基因具有植物激素、非生物胁迫、时空表达和生物进程相关的顺式作用元件。转录组数据和qRT-PCR结果显示,CsCESA家族基因在不同组织中差异表达,并且大部分基因受非生物胁迫和植物激素的诱导表达,其中CsCESA5、CsCESA20和CsCESA28均能响应低温、盐胁迫、ABA和MeJA的诱导,推测这3个基因可能在黄瓜响应非生物胁迫中发挥重要作用。Cellulose synthase(CESA)is an important enzyme in the process of cellulose synthesis,and plays very important role in cell wall formation and plant growth and development.This experiment identified and gained 35 CESA gene family members from‘9930’genome-wide data of cucumber cultivar,and divided them into 6 groups by phylogenetic analysis.Subcellular localization showed that most of the members were located in the plasma membrane,and a small part was located in the Golgi apparatus.Collinearity analysis of CESA family genes among species showed that there were 15 and 12 homologous gene pairs in the CESA gene families in Arabidopsis and tomato,respectively,and 19 and 20 homologous gene pairs in CESA gene families in watermelon and melon,respectively.Analysis on cis-acting element indicated that these members of CsCESA gene family shared similar cis-acting elements,including plant hormones-responsive,abiotic stressresponsive,temporal and spatial gene expression elements and elements involved in biological processes.Transcriptome data and qRT-PCR results showed that CsCESA gene family were differentially expressed in different tissues,and most of them were induced by abiotic stress and plant hormone.Among them,CsCESA5,CsCESA20 and CsCESA28 could respond to induction of low temperature,salt stress,ABA and MeJA,suggesting that these 3 genes might play an important role in cucumber response to abiotic stress.

关 键 词：黄瓜 纤维素合成酶(CESA) 非生物胁迫 表达分析 

分 类 号：S642.2[农业科学—蔬菜学]