节瓜CqWRKY31的互作蛋白筛选与鉴定  

Screening and Identification of Interacting Proteins of Chieh-qua CqWRKY31

作　　者：闫晋强 王敏 彭庆务[1] 刘文睿[1,2] 江彪 谢大森[1,2] 何晓明 YAN Jinqiang;WANG Min;PENG Qingwu;LIU Wenrui;JIANG Biao;XIE Dasen;HE Xiaoming(Vegetable Research Institute,Guangdong Academy of Agricultural Sciences,Guangzhou 510640,Guangdong,China;Key Laboratory for Vegetables New Technology Research of Guangdong Province,Guangzhou 510640,Guangdong,China)

机构地区：[1]广东省农业科学院蔬菜研究所,广东广州510640 [2]广东省蔬菜新技术研究重点实验室,广东广州510640

出　　处：《中国蔬菜》2022年第3期65-71,共7页China Vegetables

基　　金：广东省重点领域研发计划资助项目(2020B020220003);广东省基础与应用基础研究基金-粤穗联合基金项目(2020A1515111138);财政部和农业农村部:国家现代农业产业技术体系资助项目(CARS-23-G-48);广州市科技计划项目(201904020012);广东省农业科学院农业优势产业学科团队建设项目(202103TD,202114TD);广东省蔬菜新技术重点实验室开放课题基金项目(201902)。

摘　　要：枯萎病是节瓜生产中的主要病害,节瓜抗性材料中CqWRKY31受致萎毒素镰刀菌酸(FA)胁迫诱导表达,为节瓜响应FA胁迫的正调控因子。为深入挖掘CqWRKY31的功能,本试验利用酵母双杂交技术筛选CqWRKY31的互作蛋白。试验结果显示,共得到45个与节瓜CqWRKY31有潜在互作关系的蛋白。通过进一步回转验证,发现42个蛋白与CqWRKY31在酵母中存在互作关系。对所有单克隆进行测序、Blast分析,42个蛋白包括10个SRC2或SRC2同源蛋白、3个E3泛素蛋白连接酶、6个DNA损伤诱导蛋白、3个AT-hook核定位蛋白。试验结果为解析CqWRKY31调控节瓜枯萎病抗性机制奠定了重要基础。Fusarium wilt is a main disease in chieh-qua production.CqWRKY31 was found as a positive regulator responding to fusaric acid(FA),toxin of Fusarium wilt.In order to excavate the function of CqWRKY31,this experiment screened its interacting proteins by yeast-two-hybrid technology.The results indicated that there were 45 proteins with potential interaction with CqWRKY31.Further verification of rotary experiments confirmed that 42 proteins were interacted with CqWRKY31 in yeast.All positive clones were sequenced and blast analyzed.Among these 42 identified proteins,10 SRC2 or SRC2 homologous proteins,3 E3 ubiquitin-protein ligase DIS1s,6 DNA-DAMAGE INDUCIBLE proteins and 3 AT-hook motif nuclear-localized proteins were included.These results had laid a theoretical foundation for analyzing the resistance mechanism of CqWRKY31 in regulating Fusarium wilt of chieh-qua.

关 键 词：节瓜 枯萎病 CqWRKY31 酵母双杂交 互作蛋白 

分 类 号：S436.429[农业科学—农业昆虫与害虫防治]