大蒜素对大鼠血管平滑肌细胞迁移的影响及机制研究  

Effects and Mechanism of Allicin on Migration of Vascular Smooth Muscle Cells in Rats

作　　者：李雯静 黄丽雯[1] LI Wenjin;HUANG Liwen(Tianyou Hospital Affiliated to Wuhan University of Science and Technology,Wuhan Hubei China 430064)

机构地区：[1]武汉科技大学附属天佑医院,湖北武汉430064

出　　处：《中医学报》2022年第4期824-829,共6页Acta Chinese Medicine

基　　金：湖北省卫生健康委员会资助项目(WJ2019M257);湖北省教育厅科研项目(Q20111111)。

摘　　要：目的:观察大蒜素对大鼠血管平滑肌细胞迁移的作用,并初步探讨其作用机制。方法:采用组织块贴壁法获取SD大鼠原代血管平滑肌细胞(vascular smooth muscle cell, VSMC),并将VSMC分为对照组、模型组[1 mg·L^(-1)脂多糖(lipopolysaccharide, LPS)]、大蒜素组(1 mg·L^(-1)LPS+40μmol·L^(-1)大蒜素)。细胞计数试剂盒-8(cell counting kit-8,CCK-8)检测VSMC的活力;细胞划痕实验和Transwell实验检测细胞迁移能力;Western Blot检测各组细胞核转录因子κB p65(nuclear factorκB p65,NF-κB p65)和p-NF-κB p65的水平;酶联免疫吸附试验(enzyme linked immunosorbent assay, ELISA)检测细胞培养液中肿瘤坏死因子-α(tumor necrosis factor-α,TNF-α)和白细胞介素-6(interleukin-6,IL-6)的水平。结果:CCK-8结果显示,大蒜素的浓度≤60μmol·L^(-1)时细胞活性不受影响。与对照组比较,模型组VSMC迁移能力显著增强(P<0.05),p-NF-κB p65/NF-κB p65、TNF-α和IL-6水平显著升高(P<0.05);与模型组比较,大蒜素组VSMC迁移能力明显降低(P<0.05),p-NF-κB p65/NF-κB p65、TNF-α和IL-6水平显著降低(P<0.05)。结论:大蒜素可显著抑制LPS诱导的血管平滑肌细胞迁移,其机制可能与抑制NF-κB p65蛋白磷酸化,降低炎症反应有关。Objective: To observe the effect of allicin on the migration of vascular smooth muscle cells in rats, and to preliminarily explore its mechanism of action.Methods: The primary vascular smooth muscle cell(VSMC) of SD rats were obtained by tissue block adherence method, and the VSMC were divided into control group, model group[1 mg·L^(-1)LPS lipopolysaccharide(LPS)],allicin group(1 mg·L^(-1)LPS+40 μmol·L^(-1)allicin).Cell counting kit-8(CCK-8) was used to detect the viability of VSMC.Cell scratch assay and Transwell assay were used to detect cell migration ability;Western Blot was used to detect nuclear factor κB p65(NF-κB p65) and p-NF-κB p65 levels in each group.Enzyme-linked immunosorbent assay(ELISA) to detect tumor necrosis factor-α(TNF-α) in cell culture medium and interleukin-6(IL-6) levels.Results: The results of CCK-8 showed that the cell viability was not affected when the concentration of allicin was lower than 60 μmol·L^(-1).Compared with the control group, the migration ability of VSMCs in the model group was significantly enhanced(P<0.05),the expression of p-NF-κB p65 protein in the cells was significantly increased(P<0.05),and the levels of TNF-α and IL-6 in the cell culture supernatant were significantly increased(P<0.05).Compared with the model group, the migration ability of VSMCs in the allicin group was significantly decreased(P<0.05),the protein expression of p-NF-κB p65 was significantly decreased(P<0.05),and the levels of TNF-α and IL-6 in the cell culture medium were significantly decreased(P<0.05).Conclusion: Allicin can significantly inhibit LPS-induced vascular smooth muscle cell migration, and its mechanism may be related to the inhibition of inflammatory response mediated by the NF-κB signaling pathway.

关 键 词：大蒜素 血管平滑肌细胞 细胞迁移 NF-κB p65蛋白 大鼠 

分 类 号：R285.5[医药卫生—中药学]