数字微流控封闭-开放区间的液滴两区运动  

Droplet motion between closed and open regions on digital microfluidic chips

作　　者：王鹤 陈立国[1] WANG He;CHEN Liguo(School of Mechanical and Electrical Engineering,Soochow University,Suzhou 215021,China;Mechanical Engineering Department,Henan University of Engineering,Zhengzhou 451191,China)

机构地区：[1]苏州大学机电工程学院,江苏苏州215021 [2]河南工程学院机械工程学院,河南郑州451191

出　　处：《光学精密工程》2022年第6期711-720,共10页Optics and Precision Engineering

基　　金：江苏省高等学校自然科学研究重大项目(No.17KJA460008)。

摘　　要：为了便于在数字微流控芯片上实施完整且高自动化的生化分析,在一个柔性基底上将芯片的两种常规结构加以集成,建立混合式结构,并对液滴在封闭区和开放区之间的跨区往返运动进行研究。首先,根据力平衡分析法分析液滴在两区边界处的运动特性,推导出跨越边界的条件,得到实现两区运动的优化措施。接着,在三种柔性基底上实现液滴的两区往返运动。然后,分析了封闭区上极板空间横向位置和纵向位置对液滴两区运动的影响。最后,研究了上极板厚度对液滴两区运动的影响。实验结果表明:封闭区上极板横向位置模式Ⅱ以及薄的上极板有利于实现液滴的自由跨区往返运动,而且能够降低液滴的驱动电压;对于0.8~1.2μL的液滴来说,以聚对苯二甲酸乙二醇脂(Polyethylene terephthalate,PET)塑料片材为基底的柔性芯片上封闭区极板间距控制在150~350μm内可实现液滴的双向跨区运动。实验结果证明了液滴能够在封闭-开放区间自由往返运动,混合式结构便于实现在单一芯片上的液滴操作及高自动化程度的分析检验。In order to facilitate the implementation of complete and highly automated biochemical analyses on digital microfluidic chips,two conventional configurations were integrated on a flexible substrate to establish a hybrid configuration. The motion between the closed and open regions was studied. First,the motion characteristics of a droplet at the boundary were analyzed according to the force balance analysis method. Based on this,the conditions for crossing the boundary were deduced,and optimization methods to realize two-region motion were developed. Then,the two-region reciprocating motions of the droplet were realized on three types of flexible substrates. Finally,the influence of the horizontal and vertical positions and the thickness of the top plate on the closed region on the droplet movement between the two regions was analyzed. The experimental results show that lateral position mode II and a thin top plate are conducive to the free reciprocating motions of the droplets and can reduce the driving voltage;to realize bidirectional cross-region movement of 0. 8-1. 2 μL droplets,the gap between the plates in the closed region on the polyethylene terephthalate-based flexible chip should be within 150-350 μm. The experimental results demonstrate that the droplets can move back and forth freely between the closed and open regions,and the hybrid structure facilitates droplet operation and highly automated analysis on a single chip.

关 键 词：数字微流控 介电湿润 两区运动 混合式结构 

分 类 号：TP66[自动化与计算机技术—控制理论与控制工程] TP249[自动化与计算机技术—控制科学与工程]