人参皂苷Rg1对人内皮祖细胞增殖、黏附、迁移、成管能力及PDGF分泌水平调控观察  

Effects of ginsenoside Rg1 on proliferation,adhesion,migration,tube formation ability and PDGF secretion of human endothelial progenitor cells

作　　者：谭梅鑫 熊武[2] 黄玎玲 何晶晶 于洋 兰宏伟[2] 王婷婷[2] 张熙 TAN Meixin;XIONG Wu;HUANG Dingling;HE Jingjing;YU Yang;LAN Hongwei;WANG Tingting;ZHANG Xi(College of Integrated Traditional Chinese and Western Medicine,Hunan University of Chinese Medicine,Changsha 410208,China;不详)

机构地区：[1]湖南中医药大学中西医结合学院,长沙410208 [2]湖南中医药大学第一附属医院烧伤整形外科 [3]湖南中医药大学医学院 [4]湖南省脑科医院(湖南中医药大学临床医学院)

出　　处：《山东医药》2022年第8期11-14,共4页Shandong Medical Journal

基　　金：国家自然科学基金青年基金项目(81904217);湖南中医药大学一流学科《基础医学》项目。

摘　　要：目的观察人参皂苷(GS-Rg1)对人内皮祖细胞(EPCs)增殖、黏附、迁移、成管能力及血小板衍生生长因子(PDGF)分泌水平的调控作用。方法取足月健康新生儿脐带血,培养并鉴定EPCs,将EPCs随机分为实验组和对照组,实验组给予40 mg/L的GS-Rg1,对照组给予等量PBS。培养48 h后,采用CCK-8试剂盒检测细胞增殖能力,黏附能力测定实验检测细胞黏附能力,细胞划痕实验检测细胞迁移能力,Matrigel体外成血管实验检测细胞成管能力,采用ELISA法检测细胞培养液上清中的PDGF。结果与对照组相比,实验组EPCs增殖能力增高,细胞黏附数增多,细胞绝对迁移宽度增宽,体外成管数增多,细胞培养液上清中PDGF水平增高(P均<0.05)。结论GS-Rg1能提高人EPCs的增殖、黏附、迁移、成管能力,并促进其分泌PDGF。Objective To observe the effects of ginsenoside Rg1(GS-Rg1)on the proliferation,adhesion,migration,tube formation ability and secretion of platelet-derived growth factor(PDGF)of human endothelial progenitor cells(EPCs).Methods Umbilical cord blood was collected from healthy full-term newborns,and EPCs were cultured and identified.EPCs were randomly divided into the experimental group and control group.EPCs in the experimental group were treated with 40 mg/L GS-Rg1,and EPCs in the control group were treated with the same volume of PBS.After 48 h of culture,Cell Counting Kit-8(CCK-8)was used to detect the cell proliferation ability,adhesion ability test was used to detect cell adhesion ability,cell scratch test was used to detect the cell migration ability,Matrigel angiogenesis test in vitro was used to detect the cell tube formation ability,and ELISA was used to detect PDGF in the cell culture medium supernatant.Results Compared with the control group,EPCs’proliferation ability,cell adhesion number,cell absolute migration width,tube formation number in vitro and PDGF level in the cell culture medium supernatant increased in the experimental group(all P<0.05).Conclusion GS-Rg1 can enhance the proliferation,adhesion,migration and tube formation ability of human EPCs,and promote the secretion of PDGF.

关 键 词：人参皂苷 内皮祖细胞 血小板衍生生长因子 细胞增殖 细胞迁移 血管新生 

分 类 号：R628[医药卫生—整形外科]