杵针对糖尿病周围神经病变Keap1/Nrf2/ARE通路及氧化应激的影响研究  

Effect of pestle intervention in type 2 diabetic peripheral neuropathy on Keap1/Nrf2/ARE pathway and the relationship with oxidative stress

作　　者：王芳[1] 杨慧[1] 廖顺琪 王瑶 王寒 翁夕媚 黄亚玲 WANG Fang;YANG Hui;LIAO Shun-qi;WANG Yao;WANG Han;WENG Xi-mei;HUANG Ya-ling(Hospital of Chengdu University of Traditional Chinese Medicine,Chengdu 610032,China;Chengdu University of Traditional Chinese Medicine,Chengdu 610075,China)

机构地区：[1]成都中医药大学附属医院,四川成都610072 [2]成都中医药大学,四川成都610075

出　　处：《海南医学院学报》2022年第6期425-429,共5页Journal of Hainan Medical University

基　　金：四川省中医药管理局科学技术研究专项课题(2021MS544)。

摘　　要：目的:探讨杵针治疗糖尿病周围神经病变对Kelch样ECH相关蛋白1(Keap1)/核因子相关因子2(Nrf2)/抗氧化反应元件(ARE)通路及氧化应激的影响。方法:将符合纳入标准的60例糖尿病周围神经病变患者随机分到对照组和杵针组,各30例。对照组给予常规治疗,杵针组在对照组的基础上加以杵针疗法,每周5次,共干预4周。两组治疗前后用比色法检查血清超氧化物歧化酶(superoxide dismutase,SOD)和谷胱甘肽过氧化物酶(glutathione peroxidase,GSH-Px)含量;酶联免疫吸附剂测定血清Nrf2信号通路相关因子表达水平;RT-PCR法测Keap1、Nrf2 mRNA表达。结果:与对照组比较,杵针组治疗后血清SOD、GSH-px水平明显升高,Keap1表达量减少,Nrf2表达量增加,Keap1 mRNA表达显著降低,Nrf2mRNA表达显著增加。结论:杵针可能通过调控Keap1/Nrf2/ARE信号通路使其下游抗氧化酶SOD和GSH-Px的产生增多,增强了机体抗氧化能力,从而保护和修复糖尿病周围神经病变患者受损的周围神经。Objective:To investigate the effect of pestle needle treatment on Nrf2 pathway diabetic peripheral neuropathy(DPN)of the relationship with oxidative stress in diabetic peripheral neuropathy. Methods:Patients with DPN who met the inclusion criteria were randomly divided into control and test groups with 30 patients in each group in a 1∶1 allocation ratio. Both groups were given basic treatment,and the pestle groupwas treated with needle pestle therapy 5 times a week for a total of 4 weeks of intervention. Serum SOD and GSH PX levels were examined by colorimetry before and after intervention;Serum Keap1/Nrf2/ARE signaling pathway related factors expression levels were measured by ELISA;Keap1 and Nrf2 mRNA expression was determined by RT-PCR. Results:Compared with the control group,SOD and GSH-Px in the test group were significantly increased,Keap1 expression was decreased,Nrf2 expression was increased,Keap1 mRNA expression was significantly decreased,and Nrf2mRNA expression was significantly increased. Conclusions:The pestle needle may enhance the body’s antioxidant capacity by modulating the Keap1/Nrf2/ARE signaling pathway to enhance the production of its downstream antioxidant enzymes SOD and GSH Px,thereby protecting and repairing the damaged peripheral nerves in DPN patients.

关 键 词：糖尿病周围神经病变 杵针 氧化应激 Keap1/Nrf2/ARE信号通路 

分 类 号：R246.1[医药卫生—针灸推拿学]